Tag Archive Cell preservation solution

ByAdminsoft

Advantages of cell preservation solution

Advantages of cell preservation solution

Currently, most of the protective agents for cryopreservation of cells are glycerol or dimethyl sulfoxide. In fact, these two substances can increase the permeability of cell membranes to water. In addition, slow freezing can make the water in the cell seep out of the cell and reduce the ice crystals in the cell. The formation of ice crystals reduces cell damage caused by the formation of ice crystals.

Cells should be resuscitate by a fast melting method, which can ensure that the extracellular crystals melt in a short time,

and avoid damage to the cells due to the infiltration of water into the cells to form intracellular recrystallization; non-program cooling, -80℃ Cryopreservation in a low-temperature refrigerator for up to 3 years can ensure the safety of cryopreserved cells; cryopreservable plates (cell culture plates) can be use for cryopreservation of hybridoma cells. The cell preservation solution has a diluting effect and can be separated.

A large number of effective cells embed in the mucus allows more cells of test value to be preserve, providing a sufficient number of cells to ensure the accuracy of the test results;

at the same time, the process samples can be completely remove after low-speed centrifugal filtration The mucus in the sample effectively prevents the mucus from interfering with the test results of the sample.

The cell preservation solution can be make into a uniform monolayer cell sheet after low-speed centrifugation,

which meets the inspection requirements.

summary

Cell preservation solution is a general-purpose cell cryopreservation solution that can be use to freeze cell lines of humans and various animals; cell cryopreservation is an important technical means for cell culture, introduction, preservation and ensuring the smooth progress of experiments. In cell line establishment and line establishment, it is very important to freeze the original cells in time. In the preparation process of hybridoma monoclonal antibodies,

the cryopreservation of hybridoma cells and subcloned cells obtained from each cloning is often an indispensable experimental operation.

Because when a stable cell line or a cell line that secretes antibodies is not established,

the cell culture process may cause the experiment to fail due to cell contamination,

loss of antibody secretion ability or genetic variation, etc ,

if there is no original cell Frozen storage will be abandoned because of the above-mentioned accidents.

Advantages of cell preservation solution

ByAdminsoft

What is the use of cell preservation solution

What is the cell preservation solution:

The cell preservation solution is used for the preservation and transportation of cells taken from the human body.

It is only use for in vitro analysis and testing purposes, not for therapeutic use, and it cannot be take orally or externally.

What is the function of it:

The cell preservation solution can not only maintain, but also repair the original shape and structure,

This is the basis of pathological diagnosis.

If the shape and structure change, it may cause the diagnosis result to be wrong.

Therefore,  is requir to fix the cell morphology.

It not only has a dilution effect, but also separates a large number of effective cells embedded in mucus.

Not only can it remove impurities and keep the cells in good condition, but it can also extend the sample time without performing denaturation tests.

Make more cells of test value be preserve, provide a sufficient number of cells, and provide a guarantee for the accuracy of test results.

This product is simple and easy to use, and meets all the processes from sample collection to preservation solution:

1. Before sampling, mark the relevant sample information on the label of the sampling tube.
2. Use a sampling swab (or collector) to sample.
3. After sampling, quickly put the sampling swab into the sampling tube containing the cell preservation solution, and break the part higher than the sampling tube (or put the sample collected by the collector into the sampling tube containing the cell preservation solution, Mix evenly), screw the cap of the tube tightly.

Shenzhen Huachenyang Technology Co., Ltd. will adhere to the corporate culture of “quality first, truth-seeking and innovation, honesty and law-abiding, and win-win cooperation” with the tenet of “inheriting innovation and winning by technology”, and strive to build China’s high-end biological sample collection and preservation medical products. Microbiological sample collection and preservation product industry benchmark!

ByAdminsoft

transport medium

transport medium

What is the transmission medium

Transport media are essentially solutions of buffers with carbohydrates, peptones and other nutrients (excluding growth factors)

designed to preserve the viability of bacteria during transport without allowing their multiplication.

The primary objective of the use of the transport medium is to maintain the specimen as near its original state as possible.

The transport medium not only protects the specimen, but also minimizes the overgrowth of bacteria from the collection to the processing of the specimen.

Depending on the type of organisms suspect in the sample, transport media may vary.

However, in general, transport media are classified on the basis of the physical state as semi-solid and liquid and also on the basis of their utility as bacterial or viral transport media.

What does transport media contain?

  • Contains only buffers and salt.
  • Doesn’t contain any nutritional ingredients such as carbon, nitrogen, and organic growth factors so as to prevent microbial multiplication.
  • Addition of antibiotics and other substances like glycerol may be add for transporting specimens for tissue culture.

What samples are collected in transport medium?

All types of samples that may contain pathogens but could not be process immediately requires a transport medium.

It may be Stool, urethral swabs, Nasal and throat swabs and specimens for tissue culture etc.

What does huachenyang produce? I guess you don’t know

transport mediumfo

our campany

The factory is locate in Shenzhen City, Guangdong Province, China, mainly sampling swabs, national high-tech enterprises, high-tech factories,

with 20 flocking swab production lines, daily production capacity of more than 10 million flocking swabs, nasal swabs, throat swabs, flu transport medium,

VTM kit, UTM kit, swab kit. If you are interest in our delivery medium and sampling swabs, please contact our sales staff

website:https://www.flocked-swab.com 

Given below are some examples of transport medium with their purpose:

Cary and Blair Medium: semi-solid, white color transport medium for faeces that may contain Salmonella, Shigella, Vibrio or Cam­pylobacter

Amies medium with charcoal:

Charcoal helps eliminate metabolic products of bacterial growth, which may be especially useful in the isolation of fastidious organisms like.

However it is suggested that, some other pathogens like Campylobacter can also survive in such medium.

Amies medium without charcoal:

Are ideal for the isolation of Mycoplasma and Ureaplasma

Stuarts medium:

Commonly used for transporting specimens suspecte of having gonococci.

Also use for transporting Throat, wound and skin swabs that may contain fastidious organisms.

Venkatraman Ramakrishnan (VR) medium:

Use to transport feces from suspecte cholera patients.

Sach’s buffered glycerol saline:

Used to transport faeces from patients suspecte to be suffering from bacillary dysentery.

Viral Transport Medium:

Viral Transport Medium (VTM) is ideal for diagnosis of viral infection.

Ocular, respiratory and tissue swabs can be submit in this medium.

Fluid samples such as tracheal wash specimens or peritoneal fluid should be submit as is, in sterile vials which prevent desiccation.

In the absence of a virus transport medium, not only can the swab be placed in a sterile sealed vial, but also a few drops of saline can be added to prevent drying.

Cotton, plastic, wooden handle, polyester and other synthetic cotton swabs are all acceptable.

However, calcium alginate swabs should be avoided. Bacterial transport media are not suitable for virology.

Anaerobic Transport Medium (ATM):

It is not only a mineral salt semi-solid medium containing reducing agents, but also designed to maintain the activity of anaerobic bacteria.

It contains not only buffer mineral salts, but also sodium mercaptoacetate and cysteine to provide a reducing environment.

Resazurin may also be add as a redox indicator to reveal exposure to oxygen by turning pink.

It provides an environment that can not only maintain the viability of most microorganisms without mass reproduction, but also dilute inhibitors present in clinical materials. Examples include thioglycolate broth