Blog Masonry

ByAdminsoft Posted Jul 30, 2021

How is the cervical swab used

How is the cervical swab used

Cervical swabs are also call cervical sampling swabs, disposable flocking cervical swabs, and cervical flocking swabs. In fact, they belong to disposable flocking cotton swabs, sterile sampling cotton swabs, sterile cotton swabs and disposable sterile sampling cotton swabs.

This product is suitable for the inspection of biological samples taken from the natural cavity and other parts of people of any age. Because flocking has very light thorns on the skin, before use, people who are allergic to flocking should rotate the sampling site slightly for allergy testing before using it. Also, do not apply acetic acid or iodine solution before the inspection. Finally, be sure to check whether the packaging is intact before use. However, if it is damaged, stop using it immediately and contact the dealer or manufacturer for a replacement.

Instructions

1. When sampling, if necessary, wipe off excessive secretions from the cervix with a swab, then extend the disposable sampling swab into the cervix, and gently rotate the swab 3 to 5 times clockwise during the trial period.

2. Slowly take out the disposable sampling swab, put it into the sampling tube containing the cell preservation solution, break off the tail of the excess swab at the mouth of the tube, and leave the swab head in the sampling tube.

3. Tighten, fully immerse the tip of the flocking in the vial, and tighten the cap tightly, record the patient’s name and ID number on the label and paste it on the vial, put the vial application into the specimen bag and transport it to laboratory.

Precautions

1. Too many rotations will cause dilution or loss of the sample. Make sure that the disposable sampling swab does not touch any other objects.

2. This product is a one-time use product, please do not use it multiple times.

3. If an allergic reaction occurs after use, please stop using it immediately.

4. Please dispose of it in accordance with the laws of your region.

5. It is best not to have sex within 48 hours before the examination.

6. Do not use vaginal douche or contraceptive ointment for intravaginal medication within 48 hours before the examination.

7. It is not suitable for inspection during menstruation.

How is the cervical swab used

ByAdminsoft Posted Jul 26, 2021

What are the advantages of using saliva for DNA genetic testing?

What are the advantages of using saliva for DNA genetic testing?

In theory, DNA can be extract from any cell in the body. However, the epithelial cells of the oral mucosa have the characteristics of high metabolism, rapid turnover, and easy shedding,

which can be naturally shedding into saliva. Such cells have a complete human genomic DNA sequence. And related experimental results show that the DNA from saliva and DNA from blood were use for polymerase chain (PCR) reaction,

and the sequencing results of the two were completely consistent.

Generally speaking, there are two sampling methods for genetic testing: one is anticoagulant blood, and the other is saliva collection and testing.

Relevant experiments have shown that the PCR reaction of DNA in blood and epithelial cells extracted from saliva will produce the same sequencing results.

Therefore, whether it is blood or saliva samples, the analysis results should be based on the correct sampling method. ,

There is no difference between the two.

To be precise, it should be the epithelial cells shed in the oral mucosa in the saliva, just like the “Oral swab” of the paternity test sample, do not eat 30 minutes before the sample is collected,

and rinse your mouth with water to prevent foreign bodies in the oral cavity from affecting the timeliness of the sample ,

The epithelial cells of the oral mucosa can naturally fall off into the saliva, and these all contain DNA sequences in the human genome.

Collecting saliva for babies from a young age to a few months is as applicable as a centenarian. One is convenience, but it is painless compare to collecting fingertip blood or venous blood. For the vaccinators who are all “sniffing their teeth”, collecting saliva has the characteristics of easy operation and high safety.

Generally, blood sampling is traumatic sampling, which requires professional or laboratory personnel to collect. Generally, personal collection of samples is not recommend, so it is convenient for customers to collect saliva.

Collecting saliva for testing is a conclusion drawn through practice by thousands of experimental researchers around the world. Facts have proved that saliva samples can guarantee the accuracy of genetic test results,

so there is no need to worry too much about the reliability of saliva samples. Don’t worry about its accuracy!

What are the advantages of using saliva for DNA genetic testing?

ByAdminsoft Posted Jul 5, 2021

How to standardize genetic testing sampling

How to standardize genetic testing sampling

Genetic testing can diagnose diseases and can also be used to predict disease risks. In recent years, what has been very exciting is the development of predictive genetic testing. The use of genetic testing technology can detect the risk of disease before it occurs, and prevent it early or take effective intervention measures.

There are two methods of genetic testing sampling, one is oral swab sampling, and the other is saliva sampling.

Sampling steps for oral swabs:

Note: Do not touch the sampling swab with your hands or other objects at any time. Please rinse your mouth with water before collection. Please send the sample to the appraisal as soon as possible after the sample is dry!

1. Prepare a sterilized medical swab. Before sampling, rinse your mouth with clean water, then hold the swab in one hand, stretch into the mouth, and wipe the mucosa from the inner oral cavity more than 10 times, take out the swab, and dry it in the shade in a clean place;

2. Collect the second swab in the same way, and each person must sample at least three swabs;

3. Please don’t forget to mark the samples. Pay attention to placing different samples separately and not touching each other. You can put them in different paper envelopes or insurance films, and mark them clearly.

Saliva collection steps:

Saliva collection is a collection method that has only emerged in recent years. This method is more convenient and faster, and achieves painless and non-invasive collection. The detection effect is the same as that of oral swab sampling.

1. Oral cleaning: explain the sampling process and precautions, ask customers to rinse their mouths with water, and do not eat, drink, smoke, drink or chew gum within half an hour;

2. Before spitting out saliva, relax your cheeks and rub it gently for 30 seconds to produce saliva;

3. Gently spit the saliva secreted in the oral cavity into the oval collection port of the saliva collector. When collecting saliva, you should try to avoid excessive foam;

4. Collect saliva until the layered interface between the saliva in the collection tube and the upper foam reaches the scale line shown in 2ml;

5. Evenly buckle the cover of the collector with preservation solution onto the oval collection port. There will be a slight click when buckling, and the preservation solution evenly flows into the collection tube, indicating that the cover is buckled, otherwise, you need to gently click Open the cover of the collector carefully, and then buckle it evenly again until the preservation solution evenly flows into the sampling tube;

6. Gently shake the DNA saliva collector to make the cell storage stable liquid flow into the sampling tube more safely;

7. Confirm that the layered interface between the liquid in the sampling tube and the upper foam reaches the scale line shown in 4ml;

8. Confirm that the sampling is completed without error.

How to standardize genetic testing sampling

ByAdminsoft Posted Jun 30, 2021

What is 10 in 1 mixed sampling

What is 10 in 1 mixed sampling

10 in 1 mix collection means that after 10 people are sample separately, the samples are place in the same virus sampling tube. This mixed sampling mode is suitable for large-scale testing projects and has the advantages of efficient screening and resource saving.

So where are its advantages?

Because a virus sampling tube only puts one person’s sample, the cost will be high, and the detection time is long, and it is not suitable for large-scale nucleic acid testing. In principle, such sampling is requir for high-risk areas and key populations mode.

When using the 10-in-1 mixed sampling test, the result is negative, which means that all 10 people are negative. On the contrary, once a positive or weak positive is found, follow up immediately,

a single-tube swab will be collect again for review,

and then it will be determined which of the 10 people is positive.

Therefore, the cost of 10 in 1 mix mining is relatively low. This also caused the  mode to be 10 times faster than the previous mode of using a virus sampling tube for one person’s sample.

ByAdminsoft Posted Jun 26, 2021

How to use Huachenyang oral swab 98000

How to use Huachenyang oral swab 98000

Steps for usage

Step 1: tear off the sealing paper on the oral swab packaging bag, and gently take out the sampling swab from the bag. Note that the sampling swab should not touch other items other than the inner wall of the oral cavity to avoid contamination.

Step 2: Hold the oral swab to one side of the mouth, rotate the inner wall mucosa for 10-15 times, and then move it up and down 5-10 times to wipe with moderate force. It is advisable to stick to the inner wall of the oral cavity to ensure the sampling swab The oral mucosa exfoliated cells can be dipped everywhere; so according to the same method, collect them on the other side of the inner wall of the oral cavity.

Step 3: After the collection is completed, please take the oral swab out of the mouth and put it into the sampling test tube, break off the sampling swab handle at the place with the broken mark, cover the sampling test tube cover, and finally take out the barcode and paste it on the sampling test tube cover The junction with the pipe is sealed and sent for inspection in time.

Precautions

(1) Prepare a cup of normal saline, drink about 50-100 ml of saline and rinse your mouth thoroughly for about 10-20 seconds, then spit it out.

(2) Gently tear open the packaging bag of the oral swab as much as possible, hold the handle of the oral swab with your hand (note: do not touch the head of the sampling swab with your hands and other objects), and extend the left side of the sampling stick out of the mouth The swab enters the inner wall of the water chamber (touch the inside of the cheek on the left side), and then roll it up and down with the strength of brushing (wipe 15 to 30 times).

(3) In fact, use the same method to take a second buccal swab and extend it to the right side of the inner wall of the injection cavity for sampling. Continue to scrape cell DNA samples.

(4) Not only put the collected oral swab into the cell preservation solution and break the head, put it into the sampling tube, paste the barcode, and put it in the back bag, but also write down the name, date and other relevant information.

(5) Finally, complete the sampling and send to the laboratory.

(6) Take care not to touch the sampling head with hands and other objects.

(7) Do not smoke, drink, eat, etc. 30 minutes before sampling.

(8) In fact, oral swabs are disposable products and should not be used twice. Do not use if the packaging is damaged.

Advantage

Huachenyang Nylon Flocking Swabs have undergone a large number of clinical experiments to show that, compared with ordinary sterile cotton swabs, Huachenyang Nylon Flocking Swabs have a better effect on the collection and transportation of clinical microbial specimens. This is especially true for those specimens that cannot be submitted for inspection in time and have been placed for too long.

1. Because of the unique spray-type implanting nylon fiber technology, the collection and release of specimens are increased.

2. The swab has a total length of 14.5cm, and the plastic rod has a unique breakable design.

3. The fluffy texture can collect more target analytes.

4. There is no residual specimen volume, which speeds up the process of specimen processing.

5. The swab is sterilize and individually package.

How to use Huachenyang oral swab 98000

ByAdminsoft Posted Jun 22, 2021

What is the lysate virus sampling tube

What is the lysate virus sampling tube

The lysate virus sampling tube is also call the inactivate virus sampling tube. Its preservation solution contains lysis salt and other components, which are use to lyse and inactivate the virus. It can protect the inspectors from the second infection of the virus. Inactivate virus preservation solution is mainly a virus lysis preservation solution modify from nucleic acid extraction lysis solution,

which is add with a high concentration of lysis salt,

which can quickly and efficiently lyse and inactivate the virus protein in the sample to be test, and also contains RNase Inhibitors, which can not only protect the viral nucleic acid from being degrade,

but also ensure the integrity of the nucleic acid and affect the subsequent test results. It can also be store for a relatively long time at room temperature, saving the cost of virus samples and transportation.

Features of lysate virus sampling tube (inactivated virus sampling tube):

1. Simple operation and use, no need to prepare liquid, the system contains high-efficiency virus lysis solution, the virus can be inactivate after sampling, effectively preventing the risk of secondary infection, and ensuring the safety of transportation and testing personnel.

2. Viral DNA/RNA can be store and transport at room temperature for 1 week without degradation. After extraction according to most commercial kits, the DNA or RNA obtain is of good quality and high yield,

and can complete various genetic testing and analysis experiments. , Such as PCR or qPCR, etc,

while saving transportation costs.

3. Contains nucleic acid RNase inhibitors to maximize the protection of virus nucleic acid from being degraded and greatly improve the efficiency of nucleic acid extraction. It is suitable for the collection, preservation and transportation of common virus samples such as new coronavirus, influenza virus,

hand-foot-mouth virus, etc. It can collect throat swabs, nasal swabs or tissue samples of specific parts, and the stored samples can be used for subsequent clinical nucleic acid extraction or purification test.

ByAdminsoft Posted Jun 17, 2021

What does the saliva collector do to collect saliva?

What does the saliva collector do to collect saliva?

Saliva, commonly known as saliva, is a colorless and thin liquid excreted by the oral cavity and internal glands, with a pH of 6.6 to 7.1. An adult can excrete 1000 to 1500 ml of saliva throughout the day and night.

The composition and function of human saliva: 99% of human saliva is water, follow by substances such as amylase, peroxidase, mucin, phospholipids, phosphoprotein amino acids, potassium, calcium, and magnesium. These substances have the functions of digesting food, antibacterial, and protecting gastric mucosa.

Disadvantages of saliva: Saliva can also spread diseases through the human body with germs.

What can the saliva sampled by the saliva collector be used for?

1. Check for AIDS: In fact, you can find out whether your body is infected with HIV through saliva. The method is quick and easy.

2. Predicting ovulation: Regardless of whether a woman is breastfeeding or not, the glucose content in her saliva is sharply add 5 days before ovulation and 6 days before ovulation. So this can predict the ovulation period, which is helpful for contraception or fertility.

3. Disease prevention and cancer prevention: The mucus protein in saliva can neutralize part of the gastric acid, cause precipitation, attach to the gastric mucosa, form a protective layer, prevent peptic ulcers, and not only kill or inhibit staphylococcus, typhoid bacillus, Escherichia coli, hemolytic streptococcus, etc., and also greatly reduce the toxicity of carcinogens such as aflatoxin, benzopyrene and nitrosamines, and even become harmless substances.

4. Healing wounds: Saliva is rich in nerve growth factors and epidermal growth factors,

which make them accumulate in large amounts at the injured area and repair the wound together with the proliferated capillaries. In addition, the continuous production of collagen fibers can promote hemostasis and wound healing.

The benefits of using saliva samples: Compared with blood samples, saliva samples are easier, more convenient, safer, painless, and non-invasive. The method of collecting saliva samples reduces the risk of contamination and adds the convenience of sampling and inspection. The subjects sampled by themselves. The saliva sample collected by the saliva collector can be use in the saliva preservation fluid to replace blood DNA and is widely use in epidemiological research,

characteristic medicine, drug response monitoring, genetic disease diagnosis, disease probability prediction, and healthy life counseling.

What does the saliva collector do to collect saliva?

ByAdminsoft Posted Jun 10, 2021

What is the difference between a nasal swab and a throat swab

What is the difference between a nasal swab and a throat swab

The accuracy of detecting nasal swabs and throat swabs is usually the same. The main difference lies in the sampling method and sampling site. For nasal swabs, the nasal swab is mainly use to dip the secretions on the surface of the nasal mucosa inside the nasal cavity for detection. In fact, pharyngeal swabs are collect from the oropharynx for testing to determine whether the sampler is infect with the new coronavirus.

Under standardized operations, throat swabs and nasal swabs are two methods for collecting specimens. There is no difference in essence. Both are for collecting specimens from the pharynx. The difference is that one enters from the oral cavity, and the other is from the mouth. Enter the nasal cavity.

For throat swabs, the sampler can see the sample site, but it may cause nausea, vomiting, and other reactions, and because the pharyngeal virus content is affect by diet, drinking, and swallowing actions, it will affect it to a certain extent Throat swab detection rate.

When collecting nasal swab specimens, the swab will enter the nasal cavity. If the operation is standardize, it will cause less irritation to the mucous membrane. However, because the pharynx sampling position cannot be seen, the main reliance is on the sensory resistance of the hand. For those who require more technology, the tools use are more complex. If the operation is improper, it will cause some irritation to the nasal mucosa. Therefore, it is not recommended to do nasal swabs for patients with sinusitis and allergic rhinitis to avoid damage to the nasal cavity.

What is the difference between a nasal swab and a throat swab

ByAdminsoft Posted Jun 5, 2021

Advantages of cell preservation solution

Advantages of cell preservation solution

Currently, most of the protective agents for cryopreservation of cells are glycerol or dimethyl sulfoxide. In fact, these two substances can increase the permeability of cell membranes to water. In addition, slow freezing can make the water in the cell seep out of the cell and reduce the ice crystals in the cell. The formation of ice crystals reduces cell damage caused by the formation of ice crystals.

Cells should be resuscitate by a fast melting method, which can ensure that the extracellular crystals melt in a short time,

and avoid damage to the cells due to the infiltration of water into the cells to form intracellular recrystallization; non-program cooling, -80℃ Cryopreservation in a low-temperature refrigerator for up to 3 years can ensure the safety of cryopreserved cells; cryopreservable plates (cell culture plates) can be use for cryopreservation of hybridoma cells. The cell preservation solution has a diluting effect and can be separated.

A large number of effective cells embed in the mucus allows more cells of test value to be preserve, providing a sufficient number of cells to ensure the accuracy of the test results;

at the same time, the process samples can be completely remove after low-speed centrifugal filtration The mucus in the sample effectively prevents the mucus from interfering with the test results of the sample.

The cell preservation solution can be make into a uniform monolayer cell sheet after low-speed centrifugation,

which meets the inspection requirements.

summary

Cell preservation solution is a general-purpose cell cryopreservation solution that can be use to freeze cell lines of humans and various animals; cell cryopreservation is an important technical means for cell culture, introduction, preservation and ensuring the smooth progress of experiments. In cell line establishment and line establishment, it is very important to freeze the original cells in time. In the preparation process of hybridoma monoclonal antibodies,

the cryopreservation of hybridoma cells and subcloned cells obtained from each cloning is often an indispensable experimental operation.

Because when a stable cell line or a cell line that secretes antibodies is not established,

the cell culture process may cause the experiment to fail due to cell contamination,

loss of antibody secretion ability or genetic variation, etc ,

if there is no original cell Frozen storage will be abandoned because of the above-mentioned accidents.

Advantages of cell preservation solution

ByAdminsoft Posted Jun 2, 2021

Nasopharyngeal Flocked Swabs, Sterile, Individually Packaged

Nasopharyngeal Flocked Swabs, Sterile, Individually Packaged、

  • Sterile
  • Individually Packages
  • 8cm (80mm) Breakpoint
  • Flocked Tip
  • Polypropylene Handle
  • iClean Nasopharyngeal Flocked Swabs

    • Sterile
    • Individually Packaged
    • Breakpoint at 8cm
    • Pack of 100 Sterile
    • FDA approved

     

    Part NO. headmaterial headwidth headthickness headlength handlematerial handlewidth handlelength Breakpoint  packing
    CY/9600 Nylon fiber 3.0mm 3.0mm 16.0mm Polypropylene  2.5mm 148.0mm 80mm 100 swabs / box

    2000 swabs / ctn