DNA Polymerase Kit

PCR, referred to as Polymerase Chain Reaction, is a molecular diagnostic method based on themechanism of semi-retained enzyme-driven DNA replication. It’s to make make numerous copies of aspecific nucleic acid fragments in vitro by enzymatic synthesis.PCR requires a DNA polymerase enzyme that makes new strands of DNA with templates of existingstrands. So DNA polymerase enzyme play key roles on the sensitivity, stability, reaction time andaccuracy of molecular detection reagents.Our PCR enzyme has better performance in host nucleic acid residue, sensitivity, thermal stability,amplification speed, amplification length by means of genes Group, site-directed mutation, specialmodification.

1. HCY™ Taq DNA Polymerase Kit (E0060K101)

HCY™ Taq DNA Polymerase is a thermostable DNA polymerase from Thermus aquaticus with

specially engineered for higher robustness and sensitivity than wild-type Taq,with a molecular weight

of 106 kDa. This enzyme exhibits the 5’→ 3′ DNA polymerase and 5’→ 3′ exonuclease activities, lacks

the3’→ 5′ exonuclease activities.The resulting PCR product can be cloned directly into a T-vector (TA

cloning) due to the addition of an adenosine (A) to the 3′ end.

Main Applications

• Polymerase Chain Reaction (PCR): This is one of the most common applications. In PCR reactions, DNA polymerase kits provide thermostable DNA polymerases, such as Taq DNA polymerase. They can catalyze DNA synthesis at high temperatures. Using the target DNA as a template and guided by primers, deoxynucleotides are added one by one to the newly synthesized DNA strand, achieving exponential amplification of DNA fragments. It can be used for gene cloning, genotyping, pathogen detection, and so on.
• DNA Sequencing: It is used for DNA sequence determination. Guided by primers, DNA polymerase adds fluorescently or radioactively labeled deoxynucleotides to the newly synthesized DNA strand according to the base sequence of the template DNA. By detecting the markers, the base sequence of DNA is determined. It is a key tool in traditional sequencing techniques like the Sanger sequencing method and some next-generation sequencing technologies.
• cDNA Synthesis: After reverse transcription reactions, the DNA polymerase in the DNA polymerase kit is used to synthesize complementary DNA (cDNA) using mRNA as a template. This is very important for studying gene expression and constructing cDNA libraries. It can be used to analyze gene expression differences in cells under different physiological states or to study gene functions at the mRNA level.
• DNA Repair and Labeling: It can be used to repair damaged DNA molecules. For example, it can simulate the intracellular DNA repair process in vitro to study DNA damage repair mechanisms. Meanwhile, DNA polymerase can also incorporate specifically labeled deoxynucleotides into DNA molecules for the preparation of DNA probes, etc., so as to detect specific DNA sequences in molecular hybridization experiments.