What materials are usually used for enzyme-free centrifuge tubes?
0enzyme-free centrifuge tubes?
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Nucleic acid research
Nucleic acid extraction and purification: When extracting DNA or RNA from cells, tissues or viruses, enzyme-free centrifuge tubes can prevent the degradation of nucleic acid molecules by residual nuclease inside the tubes, ensuring that the extracted nucleic acids have complete structure and biological activity, and providing high-quality nucleic acid templates for subsequent gene sequencing, PCR amplification and other experiments.
Nucleic acid preservation and transport: Nucleic acid samples after extraction or synthesis often need to be preserved or transported, and enzyme-free centrifuge tubes can provide a stable preservation environment to avoid nucleic acids being enzymatically degraded during preservation and transport, ensure the integrity and stability of nucleic acid samples, and maintain their biological functions.
Protein Research
Protein Extraction and Separation: When extracting proteins from cells or tissues, enzyme-free centrifuge tubes can prevent proteases from degrading the target proteins, which can help to obtain complete protein molecules and ensure that the extracted proteins maintain their natural structures and functions, which is convenient for subsequent experiments such as protein structure analysis and function verification.
Protein Crystallisation Experiment: Protein crystallisation process requires strict control of experimental conditions, the enzyme-free centrifuge tube can provide a clean and enzyme-free environment, preventing enzymes from influencing protein molecules, helping protein molecules form regular crystal structures, and providing high-quality protein crystals for the analysis of three-dimensional structure of proteins by X-ray diffraction and other techniques.
Cell Culture and Research
Cell collection and processing: In the process of cell culture, cells need to be collected for subsequent experiments, the use of enzyme-free centrifuge tubes can avoid enzyme damage to the cells and maintain the activity and integrity of the cells, which is conducive to the accurate analysis of the physiological state of the cells, gene expression, and so on.
Cell freezing and resuscitation: Enzyme-free centrifuge tubes can be used for cell freezing, preventing the enzymes in the freezing solution from adversely affecting the cells at low temperatures, ensuring the survival rate and biological properties of the cells during freezing and resuscitation, which is of great significance for the preservation of rare cell lines and cell therapy research.
Clinical Diagnosis and Testing
Sample collection and preservation: In clinical testing, such as the collection of blood, urine, cerebrospinal fluid and other samples, enzyme-free centrifuge tubes can prevent the enzymes in the samples from interfering with the detection indexes, ensure the stability of the samples, and provide reliable samples for accurate clinical diagnosis.
Pathogen detection: For the detection of viruses, bacteria and other pathogens, enzyme-free centrifuge tubes can be used for the collection and processing of samples, avoiding enzyme damage to the nucleic acids or proteins of pathogens, improving the accuracy and sensitivity of pathogen detection, and contributing to the early diagnosis and treatment of diseases.
enzyme-free centrifuge tubes?
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