Fluorescence Quantitative PCR Instrument

Features and Advantages
High sensitivity: It can detect DNA or RNA templates at very low concentrations, and even single-copy gene sequences, which is of great significance for the detection of trace pathogens and the analysis of trace gene expression.
High specificity: Through the design of specific primers and fluorescent probes, the target gene sequences can be accurately recognized and detected, effectively avoiding the appearance of non-specific amplification and false-positive results, and improving the accuracy and reliability of detection.
Real-time monitoring: monitoring the change of fluorescence signal in real time during the PCR reaction, no need to carry out electrophoresis and other subsequent tests at the end of the reaction as in the case of traditional PCR, which greatly shortens the detection time, and can obtain the dynamic information of the reaction in a timely manner, such as the efficiency of the amplification, the presence of inhibitors.
Accurate quantification: Based on the linear relationship between the fluorescence signal and the template copy number, the quantitative analysis through the standard curve can accurately determine the starting copy number or concentration of the target genes in the samples, which provides accurate quantitative data for gene expression studies, pathogen load monitoring and disease diagnosis.
High throughput: Some of the fluorescence quantitative PCR instruments have multiple reaction channels or wells, which can be used for simultaneous detection of multiple samples and improve the detection efficiency, and are suitable for large-scale screening and analysis of samples, such as the detection of pathogens in batch in clinical diagnosis, and the analysis of gene expression profiles in drug discovery and development.
Applications
Medical diagnosis: widely used in pathogen detection, such as detecting nucleic acids of viruses (e.g. new coronavirus, hepatitis B virus, hepatitis C virus, HIV virus, etc.), bacteria (e.g. Mycobacterium tuberculosis, etc.), parasites, etc., to achieve early and rapid diagnosis of diseases and monitoring of disease conditions; used in the detection of tumor-related genes, to guide the diagnosis of tumors, the selection of therapeutic options and the assessment of the prognosis; also used in the diagnosis of genetic disorders, detection of gene mutations, for genetic disease diagnosis. It can also be used for the diagnosis of genetic diseases, detecting gene mutations and providing the basis for the diagnosis of genetic diseases and genetic counseling.
Biological research: It can be used to study the changes of gene expression level, to understand the mechanism of gene expression regulation under the influence of different physiological states, developmental stages or environmental factors; to analyze gene mutations and polymorphisms, to explore the relationship between gene variations and the occurrence of diseases and differences in biological traits; and it can also be used to study the regulation of the transcription level, the variation of gene copy number and other biological processes.
Food safety: Detect foodborne microorganisms in food, such as Salmonella, E. coli, Listeria monocytogenes, etc., to ensure the microbiological safety of food; detect genetically modified ingredients in food to ensure that the labeling and regulation of genetically modified food meets the requirements; it can also be used for detecting allergens and other hazardous substances in food, to safeguard the health rights of consumers.
Environmental monitoring: Detecting pathogens and pollutants in water, soil and air, assessing the degree of environmental pollution and ecological risk, such as detecting pathogenic bacteria, viruses and parasites in water, etc., providing scientific basis for environmental protection and public health.