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HPV (Human Papillomavirus) collection kit is a medical device combination specifically used to collect human samples for HPV virus detection. The following is a detailed introduction:
Components
- Sampling swab: Usually composed of a plastic rod and a sampling material at the head. The sampling material is generally soft nylon fibers or polyester fibers, which have good water absorption and flexibility, and can effectively collect sufficient epithelial cell samples without damaging the sampling site.
- Cell preservation solution: It is a special liquid medium, the main components of which include buffer, salts, proteins, etc. Its role is to stabilize the cell state in time after sample collection, prevent cell rupture and nucleic acid degradation, and ensure that the HPV virus DNA or RNA in the sample remains intact and active for a certain period of time, so as to facilitate subsequent detection and analysis.
- Collection tube: It is generally made of plastic, with good sealing and corrosion resistance. The collection tube is usually marked with graduations to facilitate the observation of the sample collection volume, and is equipped with a screw cap or a push cap to ensure that the sample does not leak during collection and transportation.
Sampling method
- Sampling site: The main sampling sites are the cervix in women and the urethral orifice, glans penis in men. For women, the doctor will use a vaginal speculum to expose the cervix, and then gently insert the sampling swab into the cervical orifice, rotate it several times to ensure that sufficient cervical epithelial cells are collected. For men, the sampling swab is gently wiped at the urethral orifice or glans penis.
- Sample processing: After the collection is completed, the sampling swab is placed in the cell preservation solution, the swab rod is broken, and the sampling head is left in the preservation solution, and then the collection tube cap is tightly screwed on, and the collection tube is shaken gently to fully mix the sample and the preservation solution.
Application scenarios
- Cervical cancer screening: HPV virus infection is one of the main causes of cervical cancer. By collecting cervical samples with the HPV collection kit and detecting HPV, it is possible to detect HPV infection at an early stage, which is of great significance for the prevention and early diagnosis of cervical cancer.
- Diagnosis of condyloma acuminatum: When condyloma acuminatum and other lesions occur in the genital area of men and women, collecting samples from the lesion site for HPV detection can help clarify the cause and guide the treatment.
- Surveillance of sexually transmitted diseases: HPV is a common sexually transmitted virus, and the HPV collection kit can be used for routine surveillance of sexually transmitted diseases to detect HPV infection in a timely manner and take corresponding prevention and treatment measures.
Precautions
- Preparation before sampling: Before sampling, sexual life, vaginal irrigation or the use of vaginal suppositories should be avoided to avoid affecting the sampling results. At the same time, the sampler should strictly follow the principle of aseptic operation to ensure the safety and accuracy of the sampling process.
- Sample transportation and preservation: The collected samples should be transported to the laboratory for testing as soon as possible within the specified time. During the transportation process, the samples should be kept at low temperature and stable to avoid severe vibration and direct sunlight. In the laboratory, the samples should be stored as required, generally in a 4℃ refrigerator for short-term storage, and in a -20℃ or -80℃ refrigerator for long-term storage.
- Interpretation of test results: The results of HPV testing may be false positive or false negative. Therefore, when interpreting the test results, a comprehensive analysis should be conducted in combination with the patient’s clinical symptoms, medical history, and other factors. If necessary, further tests and diagnoses should be performed.