HPV sampling kit is a tool kit used to collect human samples to detect whether human papillomavirus (HPV) is infected. Here is its detailed introduction:

Components

• Sampling swab: Usually made of soft fiber materials such as polyester and nylon. The head is specially designed with bristle-like or spiral shapes to facilitate the collection of epithelial cells from parts such as the cervix, vagina, and anus. The rod of the swab is generally made of plastic material with a moderate length for easy operation.
• Cell preservation solution: It is a liquid containing special components. The main function is to preserve the collected cells and prevent them from degrading or being contaminated by the outside world. The preservation solution usually contains buffer agents, proteins, antibacterial agents and other components, which can maintain the activity and integrity of cells and ensure the accuracy of subsequent detection.
• Sample collection tube: Generally a small test tube made of plastic material, used to hold the sampling swab and cell preservation solution. The collection tube usually has clear scale markings for easy observation of the sample collection volume, and is equipped with a sealing cap to ensure that the sample will not leak during transportation and storage.
• Other accessories: May also include auxiliary supplies such as disposable gloves, labels, and instructions. Disposable gloves are used to protect operators and sampled persons during the sampling process to avoid cross-infection; labels are used to identify sample information such as sampling time, sampling site, and name of the sampled person; the instructions detail the usage method and precautions of the sampling kit.

Sampling method

• Cervical sampling: During sampling, the sampled person needs to lie on the examination bed supine with legs apart. The doctor will use a vaginal speculum to expose the cervix, and then gently insert the sampling swab into the cervical opening and rotate it 3 to 5 times to ensure that enough cervical epithelial cells are collected. After collection, put the swab into the cell preservation solution, break the swab rod so that the head of the swab remains in the preservation solution, and close the cap of the collection tube tightly.
• Vaginal sampling: The sampled person also needs to take the supine position. The doctor inserts the sampling swab into the vagina and gently wipes or rotates on the vaginal wall to collect vaginal epithelial cells. During the collection process, pay attention to moderate strength to avoid damaging the vaginal mucosa. After collection, handle the sample according to the subsequent operations of cervical sampling.
• Anal sampling: For sampling at the anal site, the sampled person generally takes the lateral position or prone position. The doctor inserts the sampling swab 2 to 3 centimeters into the anus and gently rotates or wipes the epithelial cells in the anus. Then put the swab into the preservation solution and handle it in the same way as described above.

Scope of application

• Cervical cancer screening: HPV infection is one of the main causes of cervical cancer. By regularly using HPV sampling kits for detection, HPV infection can be detected early, intervention and treatment can be carried out in time, and the risk of cervical cancer can be reduced.
• Diagnosis of condyloma acuminatum: For patients suspected of having condyloma acuminatum, collecting epithelial cells around the lesion site for HPV detection can help clarify the diagnosis and guide the formulation of treatment plans.
• HPV infection monitoring: For patients who have been diagnosed with HPV infection, regularly using sampling kits for detection can monitor the infection status of the virus, changes in virus load, etc., evaluate the treatment effect, and adjust the treatment plan.

Precautions

• Before sampling: The sampled person should avoid having sexual intercourse, vaginal douching or using vaginal suppositories within 24 to 48 hours before sampling to avoid affecting the sampling results. The sampling time is best selected within 3 to 7 days after menstruation is clean.
• During sampling: Sampling personnel should conduct sampling in strict accordance with operating procedures to ensure accurate sampling sites and moderate sampling strength to avoid damage or cross-infection during the sampling process.
• After sampling: Samples should be sent for inspection in time. Generally, they are required to be delivered to the laboratory for detection within 24 hours after collection. During transportation, ensure that the sample preservation tube is well sealed and avoid violent vibration and high temperature.