Rapid nucleic acid detection reagents for monkeypox virus have many advantages, which are mainly reflected in the following aspects:

Nucleic acid detection reagents can detect extremely low concentrations of monkeypox virus nucleic acid. Even in the early stage of infection when the virus is still in the stage of low – level replication, it may be detected, effectively identifying early infected individuals and gaining precious time for epidemic prevention and control. For example, real – time fluorescent quantitative PCR reagents can detect as few as several copies of the virus nucleic acid in a sample, greatly improving the detection rate of the monkeypox virus and avoiding missed diagnoses.

Nucleic acid detection reagents are designed based on the unique nucleic acid sequences of the monkeypox virus. They will not cross – react with the nucleic acid sequences of other viruses or the human body itself, and can accurately identify the monkeypox virus. The specificity is usually 95% or even higher, thus reducing the occurrence of false – positive results and providing a reliable basis for clinical diagnosis.

Some rapid nucleic acid detection reagents, such as those based on isothermal amplification technology, can complete the detection within 30 minutes to 1 hour. Compared with traditional methods such as virus culture, the detection time is greatly shortened, which is helpful for quickly diagnosing and isolating patients and taking timely prevention and control measures to prevent the further spread of the virus.

During the incubation period of monkeypox virus infection or before the appearance of symptoms, the virus nucleic acid has already started to replicate in the body. Nucleic acid detection reagents can detect the virus at this stage, which is helpful for detecting the infection in the early stage of the disease or even in the subclinical stage. It is of great significance for the early monitoring and prevention of the epidemic, and can effectively control the spread range of the epidemic.

Reagents like real – time fluorescent quantitative PCR can not only determine whether the monkeypox virus nucleic acid exists in the sample, but also conduct a quantitative analysis of the virus nucleic acid. By detecting the intensity of the fluorescent signal and other parameters, the viral load can be accurately obtained. This has important guiding value for evaluating the severity of the patient’s condition, monitoring the treatment effect, and judging the prognosis of the patient.

Nucleic acid detection reagents are applicable to a variety of sample types, such as pharyngeal swabs, nasal swabs, skin blister fluid, blood, etc., which can meet the detection needs in different scenarios. They can play an important role in various places, whether in clinical diagnosis in hospitals, community screening, or in centralized detection points for epidemic prevention and control. They are convenient for detecting different populations and different infected parts.