Real-time Fluorescent Quantitative PCR (qPCR) has many specific applications in the field of pet detection. The following are some of the main aspects:

Virus Detection: It can be used to detect common viruses in pets, such as canine distemper virus, canine parvovirus, feline panleukopenia virus, feline herpesvirus, etc. By performing real-time fluorescent quantitative PCR detection on samples such as the pet’s blood, feces, and secretions, it is possible to quickly and accurately determine whether the pet is infected with a virus and the viral load of the infection, which is helpful for early diagnosis and timely treatment.
Bacteria Detection: It is capable of detecting pathogenic bacteria in pets, such as Escherichia coli, Salmonella, Staphylococcus, etc. When a pet shows symptoms such as diarrhea and fever, this technology can be used to determine whether it is a bacterial infection and monitor the change in the number of bacteria in the body, providing a basis for clinical medication.
Parasite Detection: For common parasites in pets, such as Toxoplasma gondii and coccidia, real-time fluorescent quantitative PCR can also play an important role. It can detect whether a pet is infected with parasites and monitor the elimination of parasites during the treatment process to evaluate the treatment effect.

Diagnosis of Genetic Diseases: Many pet breeds have specific genetic diseases, such as hip dysplasia in dogs and polycystic kidney disease in cats. Real-time fluorescent quantitative PCR can detect gene mutations or gene deletions related to these diseases, helping pet owners and veterinarians to understand the pet’s health risks in advance and take corresponding preventive and treatment measures.
Genotyping: In the field of pet breeding, real-time fluorescent quantitative PCR can be used for genotyping to help select appropriate breeding pairs, avoid passing on harmful genes to the next generation, and improve the quality of the pet breed. For example, for some pet breeds with specific coat colors or characteristics, through genotyping, the coat color and other genetic characteristics of the offspring can be accurately predicted.

Tumor Marker Detection: Real-time fluorescent quantitative PCR can detect the expression levels of tumor marker genes in the pet’s blood or tissues, such as genes related to carcinoembryonic antigen (CEA), alpha-fetoprotein (AFP), etc. When the expression levels of these genes are abnormally high, it may indicate that the pet is at risk of having a tumor, which is helpful for the early screening and diagnosis of tumors.
Tumor Treatment Monitoring: During the process of a pet receiving tumor treatment, by monitoring the expression changes of specific genes in tumor cells through real-time fluorescent quantitative PCR, the treatment effect can be evaluated, and the treatment plan can be adjusted in a timely manner. If the treatment is effective, the expression levels of genes related to tumor cells usually decrease; conversely, if the gene expression levels continue to increase or do not change significantly, it may be necessary to change the treatment method.

Evaluation of Vaccine Immune Effect: After a pet is vaccinated, by detecting the expression of immune-related genes through real-time fluorescent quantitative PCR, such as the expression levels of cytokine genes like interleukin and interferon, it is possible to evaluate whether the vaccine has induced an effective immune response. This helps to determine whether the vaccination is successful and whether booster immunization is required.
Diagnosis of Immune Diseases: When a pet suffers from immune diseases, such as systemic lupus erythematosus and rheumatoid arthritis, real-time fluorescent quantitative PCR can detect gene changes related to immune disorders, assisting veterinarians in diagnosis and disease monitoring, and providing a basis for formulating personalized treatment plans.