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The components of the preservation solution in the iClean® Viral Transport System may include the following

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  1. Buffers: These maintain the relatively stable pH value of the preservation solution, providing a suitable living environment for viruses and preventing virus inactivation due to pH changes. For example, common phosphate buffers can stabilize the pH value of the preservation solution within a certain range, ensuring the stability of the nucleic acid and protein structures of viruses.
  2. Virus – lysing agents (for inactivated types): If it is an inactivated – type preservation solution, it usually contains components such as guanidine salts. Guanidine salts are commonly – used protein – denaturing agents that can destroy the protein shells on the virus surface, thereby making the virus lose its activity and infectivity.
  3. Bacteriostatic agents: These inhibit the growth of microorganisms such as bacteria and fungi, preventing them from contaminating samples and affecting virus test results. For example, imidazolidinyl urea, which may also be added in commonly – used facial masks and lotions, poses little risk at low – dose exposure.
  4. Nutrients (for non – inactivated types): In non – inactivated – type preservation solutions, there may be nutrients required for virus survival, such as salts, proteins, and glucose, which can maintain virus activity to a certain extent for subsequent virus culturing and isolation.
  5. Indicators: Such as phenol red. It is red in a neutral environment, turns purple in an alkaline environment, and yellow in an acidic environment. Color changes can directly reflect pH changes of the preservation solution and whether it is contaminated by bacteria.
  6. Other protective agents: Some cryoprotectants, glycerol, and other components may be added to protect the structures and functions of viruses, keeping them relatively stable under different conditions such as low or normal temperatures.
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