Diagnosis of Infectious Diseases: It can quickly and accurately detect the DNA or RNA of pathogens, such as the COVID-19 virus, hepatitis B virus, and human immunodeficiency virus. It can achieve detection even when the pathogen content is low in the early stage of infection, providing a basis for the early diagnosis and treatment of diseases.
Early Diagnosis of Tumors: By detecting mutations or abnormal expressions of tumor-related genes, such as EGFR gene mutations in lung cancer and KRAS gene mutations in colorectal cancer, it helps in the early detection and diagnosis of tumors and improves the cure rate of patients.
Diagnosis of Genetic Diseases: For some genetic diseases, such as thalassemia and hemophilia, it can detect deletions, mutations, etc. of related genes, providing a basis for disease diagnosis and genetic counseling.
Disease Monitoring
Monitoring of Tumor Treatment: During the process of tumor treatment, it can monitor changes in the number of tumor cells and the expression levels of tumor-related genes to evaluate the treatment effect and adjust the treatment plan in a timely manner. For example, during chemotherapy, by detecting changes in the DNA content of tumor cells, the apoptosis and proliferation of tumor cells can be judged.
Assessment of Infectious Disease Conditions: It can dynamically monitor the pathogen load to understand the progress of the disease and the response to treatment. For example, in the treatment of AIDS, the HIV viral load is monitored to guide the adjustment of the antiviral treatment plan.
Drug Research and Development
Evaluation of Drug Efficacy: In the process of drug research and development, the fluorescence quantitative PCR instrument is used to detect the impact of drugs on the expression of target genes to evaluate the treatment effect of drugs. For example, in the research and development of anti-tumor drugs, the inhibitory effect of drugs on the expression of specific genes in tumor cells is detected to judge the effectiveness of the drugs.
Study of Drug Mechanisms: By detecting changes in the expression of related genes in cells after drug treatment, a deeper understanding of the drug’s mechanism of action can be gained. For example, by studying the impact of a certain hypoglycemic drug on the expression of genes related to the insulin signaling pathway, the hypoglycemic mechanism of the drug can be revealed.
Gene Expression Research
Research on the Mechanisms of Disease Occurrence: Analyzing differences in gene expression under disease conditions helps to reveal the mechanisms of disease occurrence and development. For example, in the study of Alzheimer’s disease, by detecting changes in the expression of related genes in the brain tissues of patients, the pathogenesis of the disease can be explored.
Research on Cell Differentiation and Development: During cell differentiation and individual development, the fluorescence quantitative PCR instrument is used to detect the expression of genes at different stages to understand the roles of genes in cell differentiation and development. For example, by studying the expression of stage-specific genes during embryo development, the molecular mechanisms of embryo development can be revealed.
The fluorescence quantitative PCR instrument plays an important role in medical research and provides powerful technical support for disease diagnosis, treatment, and research.
HUACHENGYANG
