The monkeypox virus rapid detection reagent (droplet digital PCR) has the following advantages:

2025/01/08Class Product monkeyvirus Monkeypox Virus Rapid Detection00

High specificity and sensitivity: It can significantly improve the specificity and sensitivity of detection, effectively identify the nucleic acid of the monkeypox virus at extremely low concentrations. The limit of quantification can reach as low as 5 copies per microliter. It can accurately distinguish the monkeypox virus from other viruses. In a complex sample environment, it can precisely target the nucleic acid of the monkeypox virus without being misled by other similar viruses or nucleic acids.
Accurate absolute quantification: It does not rely on internal references and standard curves and can directly provide the absolute quantification results of the monkeypox virus nucleic acid in samples. This simplifies the operation process and avoids the problem of the accuracy of results being affected by various factors during the establishment of internal references and standard curves, making the data more intuitive, accurate, and reliable.
Strong anti-interference ability: It adopts the design of independent and closed micro-reaction units, dividing the sample into multiple tiny reaction units. Each droplet is an independent and closed system, which can effectively avoid the mutual interference between PCR inhibitors and amplification products of different nucleic acid molecules, thereby improving the accuracy and reproducibility of detection.
Fast detection speed: The entire detection process usually only takes about 50 minutes. Compared with traditional detection methods, it greatly shortens the detection time and can quickly obtain detection results, thus buying precious time for the timely diagnosis and treatment of patients as well as epidemic prevention and control.
Wide adaptability to samples: It is applicable to various sample types, including rashes, crusts, vesicle fluid, pustule fluid, and whole blood. Doctors can choose the most appropriate sample for detection according to the specific symptoms and conditions of patients, improving the accuracy and timeliness of diagnosis.
Strong compatibility: It can be used not only on the digital PCR platform but also on ordinary fluorescence quantitative PCR instruments. For example, it can also be used on the ABI7500 fluorescence quantitative PCR instrument, and the limit of quantification on this instrument is 50 copies per microliter. This expands the applicable range of the kit, enabling more laboratories to conduct efficient monkeypox virus detection using existing equipment.
Good result stability: The droplet generation of positive sample liquid is good, ensuring the effectiveness of detection. The 1D scatter plot of positive samples shows a clear separation between positive signals and negative signals, avoiding the common “rain – drop” effect and guaranteeing the accuracy and stability of the results.