HUACHENGYANG
Rapid Pet Testing: Diverse Testing Items, Providing Comprehensive Care for Pet Health
1Rapid Pet Testing
View detailsThe principles of rapid pet testing mainly rely on antigen-antibody reactions, biochemical reactions, and molecular biology techniques. Here is a detailed introduction:
- Antigen-Antibody Reaction
- Many rapid test strips for infectious diseases use this principle. The test strips contain specific antibodies against the antigens of certain pathogens. When the pathogen antigens in the pet’s sample (such as blood, feces, saliva, etc.) come into contact with the antibodies on the test strip, a specific binding reaction will occur.
- Take the rapid test strip for canine distemper as an example. The test strip has antibodies against the antigen of the canine distemper virus fixed on it. After the antigen of the canine distemper virus in the pet’s sample binds to these antibodies, the result is determined through a color reaction on the test strip. If there is a sufficient amount of canine distemper virus antigen in the sample, an antigen-antibody complex will be formed, triggering a color reaction and showing a positive result; otherwise, it will be negative.
- Biochemical Reaction
- This is used to detect some physiological indicators of pets, such as blood glucose, blood lipids, liver and kidney functions, etc. The detection reagents contain components that can undergo specific chemical reactions with corresponding biochemical substances.
- Taking blood glucose detection as an example, the test strip contains substances such as glucose oxidase. When the glucose in the blood comes into contact with the glucose oxidase on the test strip, an oxidation-reduction reaction will occur, and the resulting product will cause a color change on the test strip. By reading the value corresponding to the color change of the test strip with a blood glucose meter, the blood glucose content of the pet can be obtained.
- Molecular Biology Techniques
- Some high-end rapid pet tests will use molecular biology techniques, such as the polymerase chain reaction (PCR). This technique can specifically amplify the nucleic acid fragments of pathogens in the pet’s sample.
- For example, when detecting a pet’s infection with the parvovirus, first extract the nucleic acid from the pet’s fecal sample, and then use primers specific to the nucleic acid sequence of the parvovirus for PCR amplification. If the parvovirus is present in the sample, its nucleic acid will be amplified, and subsequent detection methods (such as gel electrophoresis, fluorescence quantitative detection, etc.) can be used to determine whether there is an infection and the degree of infection.
