for automatic extraction, M002 not includes Protein K, recommend for Covid-19 virus
Specific Applications
High-throughput Gene Detection: In large-scale gene sequencing and genotyping experiments, the 96-well pre-loaded kit can process 96 samples simultaneously. Researchers only need to add the collected biological samples into the corresponding wells, and then a series of operations such as nucleic acid extraction and amplification can be carried out subsequently. A large amount of gene information of samples can be obtained in one experiment, which greatly improves the efficiency of gene detection. For example, in genome-wide association studies (GWAS), researchers analyze the genes of a large number of people to look for genetic variations related to diseases. Using the 96-well pre-loaded kit can quickly process numerous samples and accelerate the research process.
Clinical Disease Screening: In the field of clinical diagnosis, it can be used for large-scale screening of common diseases. For instance, in the screening of neonatal genetic diseases, by using the 96-well pre-loaded kit, the blood samples of multiple newborns can be tested for genes related to various genetic diseases at the same time. In terms of infectious disease screening, taking the detection of the COVID-19 virus as an example, the 96-well pre-loaded kit can be used to detect the samples of multiple suspected patients simultaneously, and quickly determine the infection status through nucleic acid testing, facilitating timely isolation and treatment.
Drug Research and Development and Screening: Pharmaceutical companies need to test the activities of a large number of compounds when developing new drugs. The 96-well pre-loaded kit can be used in cell experiments. Different cell lines are inoculated into the wells of the plate, and then various drugs or compounds to be tested are added. By observing changes in cell growth, metabolism, etc., drugs with potential therapeutic effects can be screened out. This high-throughput screening method can significantly shorten the drug development cycle and reduce development costs.
Characteristics
Pre-assembled Design: The reaction reagents, consumables, etc. in the kit have been pre-loaded into the 96-well plate according to a specific layout and proportion in advance, reducing the steps for experimenters to manually prepare reagents, and lowering the risks of operational errors and contamination. For example, in a nucleic acid detection kit, the primers, enzymes, buffers, etc. required for PCR reactions have been pre-packaged in each well, and experimenters only need to add samples to start the reaction.
Standardized Process: The reaction conditions and reagent compositions in each well are standardized, ensuring the consistency and reproducibility of experimental results. No matter which laboratory uses it, as long as the experiment is carried out according to the operation instructions, relatively stable results can be obtained. This is especially important for multi-center clinical trials and large-scale scientific research cooperation projects, ensuring the comparability of data among different research institutions.
High Compatibility: The specification of the 96-well plate is an international universal standard and is compatible with most laboratory automation equipment, such as automatic pipettes, centrifuges, microplate readers, etc. This enables the automation of experimental operations, further improving experimental efficiency and reducing errors caused by manual operations.
Advantages
High Efficiency: It can process 96 samples at a time. Compared with traditional single-sample or small-sample processing methods, it saves a great deal of time and labor costs. For example, in gene expression profiling analysis experiments, using the 96-well pre-loaded kit can complete the detection of a large number of samples within one day, while the traditional method may take several days.
Accuracy: The features of pre-assembly and standardization reduce errors caused by human operations, making experimental results more accurate and reliable. In clinical diagnosis, accurate test results are crucial for disease judgment and the formulation of treatment plans, and the 96-well pre-loaded kit can meet this requirement.
Precautions
Sample Processing: Before adding samples, it must be ensured that the quality and concentration of the samples meet the requirements. For cell samples, the activity and quantity of cells should be guaranteed; for nucleic acid samples, degradation and contamination of nucleic acids should be avoided. Meanwhile, the amount of samples added should be strictly in accordance with the instructions. Too much or too little may affect the experimental results.
Storage Conditions: The kit should be stored under the specified temperature and humidity conditions, avoiding direct sunlight and repeated freezing and thawing. Some reagents in the kit are sensitive to temperature, such as enzyme reagents. Improper storage conditions may lead to a decrease in their activity and thus affect the experimental effect.
Operation Specifications: During the use process, the operation procedures should be strictly followed to avoid cross-contamination. For example, when aspirating different samples or reagents, the pipette tips should be changed. During the centrifugation operation, it is necessary to ensure the balance of the well plate to prevent liquid overflow.