DNA Polymerase Kit

PCR, referred to as Polymerase Chain Reaction, is a molecular diagnostic method based on themechanism of semi-retained enzyme-driven DNA replication. It’s to make make numerous copies of aspecific nucleic acid fragments in vitro by enzymatic synthesis.PCR requires a DNA polymerase enzyme that makes new strands of DNA with templates of existingstrands. So DNA polymerase enzyme play key roles on the sensitivity, stability, reaction time andaccuracy of molecular detection reagents. Our PCR enzyme has better performance in host nucleic acid residue, sensitivity, thermal stability, amplification speed, amplification length by means of genes Group, site-directed mutation, special modification.

1. Polymerase Chain Reaction (PCR)
   • Gene Cloning and Amplification: In molecular biology laboratories, DNA polymerase kits are a crucial component of PCR technology. Through PCR, specific DNA fragments can be amplified exponentially. For example, in gene cloning experiments, when researchers want to insert a target gene (such as a gene encoding a specific protein) into a vector, they first need to use a DNA polymerase kit to perform PCR amplification to obtain a sufficient amount of the target gene fragment. It’s like making multiple copies of a “genetic key” to provide materials for subsequent genetic engineering operations.
   • Gene Detection and Diagnosis: In the field of medical diagnosis, it is used to detect gene mutations related to diseases. For instance, when detecting certain genetic diseases (such as cystic fibrosis, thalassemia, etc.), specific primers are designed, and PCR reactions are carried out using DNA polymerase to amplify the DNA fragments containing mutation sites. Then, the amplified products are analyzed to determine whether there are gene mutations. This method can quickly and sensitively detect tiny changes in genes and is helpful for the early diagnosis of diseases.
   • Forensic Identification: In crime scene investigations, DNA polymerase kits are used to amplify trace DNA samples. Biological samples such as hair, blood, and saliva collected from crime scenes often contain very small amounts of DNA. Through PCR technology and DNA polymerase, these trace amounts of DNA can be amplified in large quantities for subsequent DNA typing and individual identification. This plays a vital role in identifying suspects or victims.
2. DNA Sequencing
   • Sanger Sequencing: This is a classic DNA sequencing method. DNA polymerase plays a central role in the Sanger sequencing process. It uses single-stranded DNA as a template and, guided by primers, incorporates fluorescently labeled dNTPs (deoxyribonucleotides) into the newly synthesized DNA strand. Since different dNTPs have different fluorescent colors, the base sequence of DNA can be read through electrophoresis separation and fluorescence detection. This method played a huge role in the early stages of the Human Genome Project and is still widely used in sequencing small genomes or specific gene regions.
   • Application in Next-Generation Sequencing (NGS) Technologies: Although NGS technologies have their own complex sequencing processes, DNA polymerase is also indispensable. For example, in some NGS platforms based on Sequencing-by-Synthesis, DNA polymerase participates in the DNA strand synthesis reaction and determines the DNA sequence by detecting the signals generated when each base is incorporated. NGS technologies can sequence a large number of DNA fragments simultaneously and are widely used in whole-genome sequencing, transcriptome sequencing, and other fields. And DNA polymerase kits are the basis for ensuring the smooth progress of these sequencing reactions.
3. DNA Repair and Labeling
   • Research on DNA Damage Repair: In cell biology research, it is used to simulate and study DNA damage repair mechanisms. When a cell’s DNA is damaged by factors such as ultraviolet light and chemical substances, the cell’s internal repair mechanisms are activated. Researchers can use DNA polymerase kits to construct in vitro DNA models containing damage, add specific repair proteins and DNA polymerase, and observe how they cooperate to repair the damaged DNA. This helps to gain an in-depth understanding of the cell’s self-repair mechanisms and provides a theoretical basis for the research on diseases such as cancer, as cancer cells often have abnormal DNA repair capabilities.
   • DNA Labeling and Tracking: By adding labeled dNTPs (such as biotin, digoxin, etc.) to the DNA polymerase reaction system, the newly synthesized DNA can be labeled. This labeled DNA can be used for various experimental purposes. For example, in chromosome mapping experiments, the labeled DNA can hybridize with specific regions on the chromosome to determine the position of genes on the chromosome; or in studies on cell proliferation and differentiation, track the distribution and changes of the labeled DNA to understand the dynamic changes of DNA within the cell.