Fluorescence quantitative PCR instrument is an important instrument widely used in many fields such as molecular biology and medicine, the following are its specific applications:

Clinical diagnosis
Pathogen detection
Virus detection: plays a key role in detecting viral infections. For example, during the New Crown outbreak, fluorescence quantitative PCR instrument was used to detect the nucleic acid of New Crown virus. By amplifying and quantifying the viral RNA in patient samples (e.g. pharyngeal swabs, nasal swabs, etc.), it was able to quickly and accurately determine whether a patient was infected with the virus or not. It is also used to detect other viruses, such as influenza virus, hepatitis B virus, hepatitis C virus, human immunodeficiency virus (HIV), etc., to help doctors in disease diagnosis and treatment monitoring.
Bacterial testing: It is used to detect bacteria that cause infectious diseases. For example, for the diagnosis of tuberculosis, the DNA of Mycobacterium tuberculosis can be detected to determine whether a patient is infected with Mycobacterium tuberculosis. In addition, it can also detect bacteria that cause sepsis, pneumonia and other diseases, providing timely and accurate diagnostic basis for clinical treatment.
Parasite detection: It is also used in the diagnosis of some parasitic infections. For example, detecting the nucleic acid of parasites such as Plasmodium can assist in the diagnosis of diseases such as malaria, especially in malaria-endemic areas, this test can quickly identify infected cases, which can help timely treatment and control the spread of the disease.
Genetic Testing and Disease Risk Assessment
Diagnosis of hereditary diseases: It is used to detect gene mutations associated with hereditary diseases. It is used to detect disease-causing genes for diseases such as cystic fibrosis, thalassaemia and Huntington’s disease. By analysing the DNA samples of patients, it can determine whether an individual carries disease-causing mutations, providing an important basis for early diagnosis of diseases, genetic counselling and family genetic risk assessment.
Tumour-related gene detection: It is widely used in the process of tumour diagnosis and treatment. It can detect the expression level or mutation of tumour-related genes, such as BRCA1 and BRCA2 genes in breast cancer and EGFR gene in lung cancer. The results of these tests can help doctors determine the risk of tumour development, select appropriate treatment options (e.g. targeted therapy) and assess the prognosis of patients.
Research Areas
Gene Expression Research
is a powerful tool for studying changes in gene expression levels in different cell types, physiological states and developmental stages. For example, researchers can detect changes in the expression of specific genes during embryonic development using fluorescence quantitative PCR to understand the role of these genes in embryo formation and organ development. In cell differentiation studies, it can also be used to analyse the differences in gene expression in cells at different stages of differentiation, thereby revealing the molecular mechanisms of cell differentiation.
For comparing differences in gene expression in different tissues or organs. For example, comparing the expression of certain genes in normal tissues and diseased tissues can help to identify potential genetic markers related to the development of diseases and provide clues for the study of disease mechanisms.
Molecular Biology Research
Play a role in the study of DNA-RNA-protein interactions. For example, it can be used to infer the regulation of gene transcription by detecting changes in the expression level of mRNA, and thus to study the interaction of transcription factors with gene promoter regions. Meanwhile, it can also be used to study the phenomenon of RNA interference (RNAi), observe the effect of specific small RNA molecules on the expression of target mRNA, and provide experimental basis for the study of gene function.
For verification in the process of gene cloning and construction. After constructing recombinant DNA molecules or gene editing, the fluorescence quantitative PCR instrument can be used to quickly verify whether the target gene is successfully inserted, whether it is correctly expressed, and whether the amount of expression is in accordance with the expectation, so as to improve the accuracy and efficiency of genetic engineering experiments.

Food Safety Testing Fields
Food Microbiological Testing
It is used to detect harmful microorganisms in food. For example, detecting nucleic acids of pathogenic bacteria (e.g. Salmonella, E. coli O157:H7, Listeria monocytogenes, etc.) and viruses (e.g. norovirus) in foods such as meat, milk, seafood, etc., in order to determine whether the food is contaminated. This detection method is more rapid and sensitive than the traditional microbiological culture method, and can effectively guarantee food safety.
It is used to monitor microbial contamination and control microbial growth during food processing. For example, during the production of fermented food, the growth of fermentation strains can be detected, while monitoring whether there is any contamination by stray bacteria to ensure food quality and production safety.
Genetically Modified Food Detection
Can detect genetically modified ingredients in food. By detecting the unique gene sequences of genetically modified crops, it can determine whether the food contains genetically modified ingredients and the content of genetically modified ingredients. This is of great significance to the labelling management and safety assessment of GM food, satisfying consumers’ demand for the right to know about food, and also helps the regulatory authorities to monitor the GM food market.