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Nucleic acid extraction kits are tools used to extract nucleic acids (DNA or RNA) from biological samples, and their characteristics usually include the following aspects:

High Efficiency

  • Rapid Extraction: It can complete the nucleic acid extraction process in a relatively short time, generally within dozens of minutes, which greatly improves the experimental efficiency. For example, some kits can complete the extraction of DNA from cell samples within 30 minutes.
  • High Yield: By adopting optimized extraction methods and reagent formulations, it can obtain a high yield of nucleic acids from various biological samples. It can ensure that a sufficient amount of nucleic acids can be extracted even from trace samples for subsequent experiments. For instance, micrograms of DNA or RNA can be extracted from a small amount of blood cells or tissue samples.

High Purity

  • Impurity Removal: It can effectively remove impurities such as proteins, polysaccharides, lipids, and other possible biomolecular contaminants, ensuring that the extracted nucleic acids have a high purity. The A260/A280 ratio is usually between 1.8 and 2.0, meeting the requirements of most molecular biology experiments.
  • No Inhibitor Residue: During the extraction process, substances that may inhibit subsequent enzymatic reactions can be completely removed, such as residual organic solvents and metal ions, ensuring that the nucleic acids can react normally in subsequent experiments like PCR, restriction enzyme digestion, and sequencing.

Simple Operation

  • Straightforward Steps: The kit is usually equipped with a detailed operation manual, and the experimental steps are relatively simple. It does not require complex equipment or professional skills. Ordinary laboratory personnel can master the operation skillfully after simple training, reducing the experimental difficulty and error.
  • Pre-dispensed Reagents: The reagents are pre-dispensed in advance, reducing the experimental preparation time and avoiding errors and contamination risks caused by reagent weighing and preparation operations. Users only need to add the corresponding reagents according to the instructions to carry out the extraction operation.

Strong Compatibility

  • Diverse Sample Types: It is applicable to a variety of biological samples, including blood, tissues, cells, saliva, feces, etc., and can also extract high-quality nucleic acids from samples of different sources, meeting different experimental needs. For example, in clinical diagnosis, it can be used for the detection of patients’ blood and tissue samples, and in scientific research, it can be used for the study of animal and plant cell samples.
  • Suitable for Multiple Nucleic Acid Types: Some kits can extract both DNA and RNA, and even can extract DNA and RNA simultaneously, providing convenience for different experimental purposes. Researchers can flexibly choose to extract different types of nucleic acids according to their experimental needs.

Good Stability

  • Stable Reagents: The reagents in the kit have good stability under the specified storage conditions and have a relatively long shelf life, generally about 12 to 24 months. This reduces the risk of experimental failure caused by reagent deterioration and ensures that consistent extraction effects can be obtained during multiple uses within the validity period.
  • High Reproducibility of Results: The strict production process and quality control system ensure the stable performance of the kit between different batches, enabling relatively consistent extraction effects and experimental results. When the same kit is used for nucleic acid extraction in different laboratories or in the same laboratory at different times, the yield and quality of the extracted nucleic acids are basically similar.

High Safety

  • Non-toxic or Low-toxic Reagents: The reagents used in the kit try to adopt non-toxic or low-toxic chemical substances, reducing the harm to the health of experimental personnel and the pollution to the environment. For example, the extraction method without phenol-chloroform is adopted to avoid the use of toxic organic solvents such as phenol-chloroform.
  • Safety Design: Safety factors are considered in the product design. For example, the reagent bottles have good airtightness to prevent reagent leakage. Some kits are also equipped with protective supplies such as gloves and masks to ensure the safety of experimental personnel.
Nucleic acid extraction kits
Nucleic acid extraction kits
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