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Nucleic acid extraction kits are tools used to extract nucleic acids (DNA or RNA) from biological samples, and they can be applied in multiple fields. The specific applications are as follows:
Pathogen Detection: Nucleic acids of pathogens can be extracted from samples such as patients’ blood, sputum, and urine to detect pathogens like the COVID-19 virus, hepatitis B virus, and Mycobacterium tuberculosis, enabling early and accurate diagnosis of diseases.
Genetic Disease Diagnosis: By extracting DNA from patients’ blood or tissue samples and analyzing abnormal conditions such as mutations, deletions, or duplications of specific genes, it can assist in diagnosing genetic diseases such as hemophilia and thalassemia, providing a basis for genetic counseling and prenatal diagnosis.
Tumor Diagnosis and Monitoring: Nucleic acids can be extracted from tumor tissues or cell-free DNA in patients’ blood to detect mutations in tumor-related genes, helping doctors choose appropriate targeted drugs and monitor the recurrence and metastasis of tumors during the treatment process.
Biological Research
Gene Expression Analysis: RNA is extracted from cell or tissue samples for studying changes in the expression levels of genes in different physiological states, developmental stages, or disease models, which helps to reveal the functions and biological mechanisms of genes.
Genomics Research: After extracting high-quality DNA, whole-genome sequencing, genotyping, and other studies can be carried out to understand the genomic structure, genetic variations, and evolutionary relationships of organisms.
Molecular Cloning and Genetic Engineering: It provides pure nucleic acid templates for gene cloning, vector construction, and other experiments, which are used to construct recombinant DNA molecules, express specific proteins, or perform gene editing operations.
Forensic Identification
Individual Identification: DNA is extracted from biological samples such as blood, hair, and saliva at crime scenes and compared with the DNA of suspects or victims to achieve individual identification, providing crucial evidence for case investigation.
Kinship Identification: By extracting the DNA of relevant persons and analyzing genetic markers at specific gene loci, kinship such as parent-child relationships and grandparent-grandchild relationships can be determined. It is commonly used in fields such as searching for missing persons and inheritance.
Food and Environmental Testing
Food Testing: It is used to detect pathogens and genetically modified components in food. For example, it can detect pathogenic bacteria such as Salmonella and Escherichia coli in meat or detect whether there are nucleic acid components of genetically modified crops in food, ensuring food safety.
Environmental Microorganism Detection: Nucleic acids of microorganisms are extracted from environmental samples such as soil and water samples to analyze the structure, diversity, and functional genes of the microbial community in the environment, monitor environmental pollution conditions, and evaluate the health of ecosystems.