Pipette tip

The pipette tip is a simple plastic moulded disposable filter-type tool that is an integral part of the pipette, and is sized accordingly for different types of pipettes.

HCY Advantages of pipette tips:

Free of deoxyribonuclease
Ribonuclease-free
Pyrogen-free
Sterility
Low retention

Correctly Choosing Pipette Tips

• Compatibility: Select pipette tips that match the brand and model of your pipette. Different brands and specifications of pipettes may vary in size and design. Only compatible tips can ensure a tight connection, preventing liquid leakage and inaccurate aspiration.
• Capacity: Choose pipette tips with an appropriate capacity according to the volume of liquid to be transferred. Common capacities of pipette tips include 10 μL, 20 μL, 100 μL, 200 μL, 1000 μL, etc. The maximum capacity of the tip should be slightly larger than or equal to the volume of the liquid to be transferred to ensure accurate transfer without introducing significant errors due to excessive capacity.
• Material: Generally, there are two types of materials: ordinary polypropylene (PP) and those with filters. Ordinary PP tips are cost-effective and suitable for routine experiments. Tips with filters can prevent liquid from being sucked back into the pipette to avoid cross-contamination. They are often used in situations where high accuracy of experimental results and freedom from contamination are required, such as PCR experiments and cell culture.
• Sterility Requirements: When conducting experiments with strict sterility requirements, such as in cell biology and molecular biology, sterile pipette tips should be selected to prevent microbial contamination of experimental samples.

Correctly Using Pipette Tips

• Installation: Hold the pipette vertically and attach the tip to the lower end of the pipette. Gently press down and rotate to ensure a tight connection between the tip and the pipette to avoid looseness.
• Aspirating Liquid: Press the pipette button down to the first stop point, insert the tip vertically into the liquid, and slowly release the button to aspirate the liquid. The depth at which the tip is immersed in the liquid should be appropriate, usually 1 – 2 mm. Avoid immersing it too deeply to prevent excessive liquid adhering to the wall of the tip or too shallowly to avoid generating air bubbles.
• Transferring Liquid: Move the tip to the target container, press the pipette button down to the first stop point, pause for a moment, and then press it down to the second stop point to ensure that all the liquid in the tip is completely discharged.
• Replacing Tips: To prevent cross-contamination, the tip should be replaced promptly after transferring each sample. Press the tip ejection button on the pipette to make the tip fall into the designated waste container. Avoid touching used tips directly with your hands.