Portable Real-Time fluorescent quantitative PCR

Precautions

1. Sample Collection and Processing

• The collection method and quality of samples are crucial to the test results. Sample collection should be carried out strictly in accordance with the corresponding operating procedures to ensure that the collected samples are representative and not contaminated. For example, when collecting samples for virus nucleic acid detection, attention should be paid to the selection of sampling sites and sampling depth to avoid collecting samples with too low a virus content or containing too many impurities. The collected samples should be processed and detected as soon as possible. If they cannot be detected in a timely manner, they should be properly stored at an appropriate temperature and conditions to prevent nucleic acid degradation.

2. Reagent Selection and Preservation

• Select reliable and stable PCR reaction reagents and fluorescence detection reagents, and store and use them strictly in accordance with the reagent instructions. Reagents should be stored under the specified temperature conditions (such as -20 °C or 4 °C), and avoid repeated freezing and thawing, otherwise, the activity and stability of the reagents may be affected, resulting in inaccurate test results. Before using the reagents, check the expiration date and appearance of the reagents. If there are any abnormalities, stop using them.

3. Instrument Calibration and Maintenance

• Regularly calibrate and maintain the portable real-time fluorescence quantitative PCR instrument to ensure its stable and reliable performance. Calibration contents include the calibration of parameters such as temperature accuracy and fluorescence detection sensitivity. Standard products and calibration instruments can be used for calibration operations. Meanwhile, keep the instrument clean and hygienic, avoid dust, water vapor, and other impurities from entering the inside of the instrument and affecting its normal operation. During the use process, if the instrument malfunctions or has abnormal conditions, stop using it immediately and contact professional technicians for repair.

4. Operation Specifications and Quality Control

• Operators should receive professional training, be familiar with the operation process and precautions of the instrument, and conduct detection operations strictly in accordance with the operating procedures to avoid errors and incorrect results caused by improper operations. During each detection process, positive controls, negative controls, and standard curve samples should be set up to monitor the quality and accuracy of the detection process. If the test results of the control samples are abnormal, the cause should be investigated in a timely manner and the detection should be repeated. In addition, the analysis and interpretation of the test results should be combined with multiple factors such as clinical symptoms and epidemiological data for comprehensive judgment, and avoid making a diagnosis conclusion based solely on a single test result.

5. Biosafety Protection

• When handling samples containing pathogens, strictly abide by biosafety protection regulations and take appropriate protective measures, such as wearing masks, gloves, protective clothing, etc., to prevent operators from being infected by pathogens. Meanwhile, properly handle the samples and waste after detection, and conduct inactivation and disinfection treatment in accordance with biosafety requirements to avoid environmental pollution and cross-infection.