Portable Real-Time Fluorescence Quantitative PCR – A Portable and Precise Detection Tool”

In today’s era of rapid technological development, portable real-time fluorescence quantitative PCR technology has become an indispensable tool in many fields due to its unique advantages.

The specific applications of portable real-time fluorescence quantitative PCR are extensive. In the medical field, it plays a crucial role in the diagnosis of infectious diseases. Taking the COVID-19 pandemic as an example, at transportation hubs such as airports and railway stations, as well as in primary medical institutions, portable devices can quickly test samples from suspected patients to determine whether they are infected with the COVID-19 virus. Through specific amplification of the viral nucleic acid and real-time fluorescence monitoring, accurate results can be obtained within just dozens of minutes, greatly improving the detection efficiency and helping to timely identify the source of infection and control the spread of the epidemic. In the detection of genetic diseases, it can detect mutations in specific genes. For example, it can screen for genes related to diseases such as thalassemia, providing strong support for genetic counseling and prenatal diagnosis, helping families understand genetic risks in advance and make reasonable decisions.

This device has remarkable characteristics, and its advantages are even more prominent. Firstly, its portability is outstanding. It is small in size, about the size of an ordinary laptop, and lightweight, making it convenient to carry to any site where testing is needed. Whether it’s a remote medical station or an epidemic prevention and control point in the wild, it can be easily deployed. Secondly, it is fast and efficient. Compared with traditional PCR technology, its rapid temperature cycling system can significantly shorten the detection time. Generally, the detection process with 40 cycles can be completed within 1 to 2 hours, allowing patients and medical staff to obtain test results more quickly and saving precious time. Thirdly, high sensitivity and specificity are its core advantages. It can accurately detect extremely small amounts of target nucleic acid sequences, effectively avoiding false-positive and false-negative results and ensuring the accuracy of diagnosis. Moreover, it has the ability of real-time quantitative analysis. It can not only detect the presence of the target nucleic acid but also accurately measure its content, providing an important basis for the assessment of disease conditions and the formulation of treatment plans.

However, there are also some precautions when using portable real-time fluorescence quantitative PCR. The sample collection process is crucial and must be carried out strictly in accordance with standard operating procedures to ensure the quality of the collected samples. Otherwise, the accuracy of the test results will be affected. The selection and storage of reagents cannot be ignored either. High-quality and stable reagents should be selected and properly stored under the specified temperature conditions to prevent reagent failure. Regular calibration and maintenance of the instrument are also essential. Professional personnel are required to operate according to standard procedures to ensure the stable and reliable performance of the instrument. In addition, operators must receive professional training and be familiar with the operation process and precautions of the device to ensure the smooth progress of the detection process.

In conclusion, portable real-time fluorescence quantitative PCR, with its many advantages such as portability, speed, and precision, plays an important role in medical diagnosis, epidemic prevention and control, and other fields, providing powerful technical support for safeguarding public health and promoting scientific research.