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To ensure the accuracy of DNA collection using gene detection sampling kits, strict control is required in multiple aspects, including before, during, and after sampling. The specific measures are as follows:
Select Appropriate Sampling Kits: Based on the purpose of detection and the type of samples, choose gene detection sampling kits that have been certified by authoritative institutions and are of reliable quality. Check the production qualifications, quality inspection reports, and other aspects of the products to ensure that the sampling kits meet the requirements in terms of preservation and transportation.
Prepare Well for Sampling: Explain the sampling process and precautions to the sampled individuals in detail. For example, before collecting oral swab samples, keep the mouth clean and avoid eating, drinking, or smoking within 30 minutes before sampling to ensure that the samples are not interfered with by food residues, oral bacteria, and so on.
Confirm the Identity of the Sampled Individuals: Strictly check the information such as the name, age, gender, and ID number of the sampled individuals to avoid misidentifications and ensure that the collected samples accurately correspond to the tested subjects.
During Sampling
Standardize Sampling Operations: Sampling personnel should receive professional training and master the correct sampling methods. For example, when collecting oral mucosal cells, swabs should be rubbed repeatedly on the inner wall of the mouth to ensure that a sufficient number of cells are obtained. When collecting blood samples, follow the principle of aseptic operation and use disposable blood collection devices to avoid sample contamination.
Control the Sampling Volume: Collect an appropriate amount of samples according to the requirements of the sampling kits. Too little may result in insufficient DNA content, affecting the test results; too much may exceed the preservation and processing capabilities of the sampling kits and also affect the accuracy.
Prevent Cross-Contamination of Samples: During the sampling process, use disposable sampling tools to avoid contact between different samples. For example, when collecting multiple samples, change gloves, swabs, and other items in a timely manner to prevent the DNA of different samples from contaminating each other.
After Sampling
Properly Preserve Samples: Place the samples in the appropriate preservation solution according to the instructions of the sampling kits and store them at the specified temperature. For example, some samples need to be stored at -20 °C or -80 °C. Ensure that the temperature of the storage equipment is stable to prevent the degradation of DNA in the samples.
Transport Samples in a Timely Manner: Choose professional logistics and transportation companies and use cold-chain transportation equipment to ensure that the temperature and environmental conditions during sample transportation meet the requirements. Meanwhile, ensure the safety during transportation to avoid damage or loss of samples.
Strictly Implement Sample Reception and Testing Processes: When the laboratory receives the samples, carefully check the sample information and examine the packaging and preservation status of the samples. During the testing process, adopt standardized experimental procedures and advanced detection technologies, such as high-throughput sequencing technology and fluorescence quantitative PCR technology, and conduct multiple repeated tests to improve the accuracy of the test results.