When using nucleic acid detection reagents for monkeypox virus, the following precautions should be taken:

2025/02/28Class Product monkeyvirus news Monkeypox Virus Rapid Detection10

Sample Collection: Collect samples strictly in accordance with the operation instructions to ensure accurate collection sites and sufficient sample volume. For example, when collecting a swab sample from a skin lesion site, wipe the lesion area thoroughly. When collecting a blood sample, pay attention to using the correct blood collection tube and anticoagulant to avoid situations such as hemolysis that may affect the test results.
Sample Storage and Transportation: The collected samples should be tested as soon as possible. If storage or transportation is required, follow the requirements specified in the reagent instruction manual. Generally, samples need to be stored and transported under specific temperature conditions, such as low-temperature refrigeration or freezing, to prevent nucleic acid degradation and ensure the accuracy of the test.
Reagent Storage and Use: Nucleic acid detection reagents should be stored under the specified temperature, humidity, and other conditions, and kept away from direct sunlight and moisture. Carefully check the packaging of the reagent before use to ensure it is intact and there is no situation of expiration or deterioration. At the same time, prepare and use the reagent strictly in accordance with the instructions in the reagent manual to avoid reagent contamination and misuse.
Operation Specification: Operators should receive professional training and be familiar with the detection process and operation specifications. During the operation, strictly comply with the laboratory biosafety regulations and wear personal protective equipment such as gloves, masks, and goggles to prevent cross-contamination of samples and infection of operators. Also, carry out operations such as sample addition, mixing, incubation, and detection according to the specified procedures to ensure accurate operation.
Quality Control: The laboratory should conduct regular internal quality control, and use known positive and negative standards or quality control products to verify the detection reagent and the detection process to ensure the accuracy and reliability of the test results. In addition, it should actively participate in external quality assessment activities, compare the results with other laboratories, and promptly identify and correct problems existing in the detection process.
Result Interpretation: Interpret the results strictly in accordance with the criteria specified in the reagent instruction manual, and do not change the interpretation criteria at will. For suspicious results or results that do not match the clinical symptoms, repeat the test or use other detection methods for verification. If necessary, make a comprehensive judgment in combination with the patient’s clinical symptoms, epidemiological history, etc.
Waste Disposal: The waste samples, reagents, consumables, etc. used during the detection process should be collected by category, disinfected, and properly disposed of in accordance with the relevant regulations for medical waste treatment to prevent the spread of the virus and environmental pollution.