DNA Polymerase Kit

PCR, referred to as Polymerase Chain Reaction, is a molecular diagnostic method based on themechanism of semi-retained enzyme-driven DNA replication. It’s to make make numerous copies of aspecific nucleic acid fragments in vitro by enzymatic synthesis.PCR requires a DNA polymerase enzyme that makes new strands of DNA with templates of existingstrands. So DNA polymerase enzyme play key roles on the sensitivity, stability, reaction time andaccuracy of molecular detection reagents.Our PCR enzyme has better performance in host nucleic acid residue, sensitivity, thermal stability,amplification speed, amplification length by means of genes Group, site-directed mutation, specialmodification.

Polymerase Chain Reaction (PCR)

• Purpose: It is used to amplify specific DNA fragments for subsequent analysis, such as gene cloning, gene expression analysis, DNA sequencing, etc.
• Principle: The DNA polymerase in the DNA polymerase kit can use DNA as a template. Under the guidance of primers, according to the principle of base complementary pairing, dNTPs are added one by one to the 3′ end of the primer to synthesize a new DNA strand. Through repeated denaturation, annealing, and extension steps, exponential amplification of the DNA fragment is achieved.
• Example: In medical diagnosis, it can be used to detect the specific gene sequences of pathogens. For example, detecting the nucleic acid of the novel coronavirus to assist in diagnosing the infection situation.

Reverse Transcription PCR (RT-PCR)

• Purpose: Reverse transcribe RNA into cDNA, and then perform PCR amplification, which is used for analyzing gene expression levels, cloning cDNA, etc.
• Principle: First, use reverse transcriptase to reverse transcribe RNA into cDNA. Then, use the DNA polymerase in the DNA polymerase kit to perform PCR amplification with cDNA as the template.
• Example: In cancer research, detect the expression level of cancer-related genes through RT-PCR to understand the role of genes in the occurrence and development of cancer, providing a basis for the diagnosis and treatment of cancer.

DNA Sequencing

• Purpose: Determine the DNA sequence, which is of great significance for gene structure and function research, genetic disease diagnosis, molecular evolution research, etc.
• Principle: In the DNA sequencing reaction, the DNA polymerase in the DNA polymerase kit uses the DNA to be sequenced as a template, incorporates dNTPs with fluorescent or radioactive labels into the newly synthesized DNA strand, and determines the DNA sequence by detecting the labels.
• Example: In the Human Genome Project, DNA polymerase kits were widely used for DNA sequencing, providing basic data for human genetic research and disease prevention and treatment.

Gene Cloning

• Purpose: Insert the target gene fragment into a vector to construct a recombinant DNA molecule, so that it can be replicated and expressed in the host cell.
• Principle: First, use the DNA polymerase kit to amplify the target gene fragment by PCR. Then, ligate it with the vector that has been digested by restriction enzymes to construct a recombinant DNA molecule. Finally, introduce the recombinant DNA molecule into the host cell.
• Example: When producing biological drugs such as insulin, use gene cloning technology to clone the insulin gene into host cells such as Escherichia coli to achieve large-scale production of insulin.

Site-directed Mutagenesis

• Purpose: Mutate the DNA sequence at a specific position, which is used to study gene function, the relationship between protein structure and function, etc.
• Principle: Design primers containing the mutation site, use the DNA polymerase kit for PCR amplification to introduce the mutation site into the DNA sequence, and then obtain the DNA molecule containing the mutation through screening and identification.
• Example: In enzyme engineering, change the amino acid sequence of the enzyme through site-directed mutagenesis to optimize the activity, stability, and other properties of the enzyme, and improve the application value of the enzyme in industrial production.