DNA Polymerase Kit

PCR, referred to as Polymerase Chain Reaction, is a molecular diagnostic method based on the

mechanism of semi-retained enzyme-driven DNA replication. It’s to make make numerous copies of a

specific nucleic acid fragments in vitro by enzymatic synthesis.

PCR requires a DNA polymerase enzyme that makes new strands of DNA with templates of existing

strands. So DNA polymerase enzyme play key roles on the sensitivity, stability, reaction time and

accuracy of molecular detection reagents.

Our PCR enzyme has better performance in host nucleic acid residue, sensitivity, thermal stability,

amplification speed, amplification length by means of genes Group, site-directed mutation, special

modification.

Gene Amplification: It is used to specifically amplify specific DNA fragments. For example, in medical diagnosis, it can amplify specific gene fragments of pathogens to detect whether there is an infection. For instance, it can detect the specific nucleic acid sequences of the novel coronavirus. In forensic identification, it can amplify the DNA fragments in biological samples from crime scenes for comparison with the suspect’s DNA.
SARS-CoV-2 Detection: During the COVID-19 pandemic, the PCR technique combined with DNA polymerase kits was widely used to detect the viral nucleic acid in patients’ samples, enabling rapid diagnosis of infected individuals.

Sanger Sequencing: It is a traditional DNA sequencing method. The enzyme in the DNA polymerase kit can synthesize a complementary strand according to the template DNA under the guidance of a primer. By incorporating fluorescently labeled ddNTPs, the DNA sequence can be determined. It is often used for gene sequence determination, gene mutation detection, etc. For example, it can sequence genes related to genetic diseases to identify pathogenic mutations.
Next-Generation Sequencing (NGS): In technologies such as next-generation sequencing, DNA polymerase also plays a crucial role. It is used for the amplification and synthesis of DNA fragments during library construction, enabling the parallel sequencing of a large number of DNA fragments. It can be applied to whole-genome sequencing, transcriptome sequencing, etc., providing massive data for studying gene expression, genetic variations, and so on.

Construction of Recombinant DNA Molecules: In gene cloning experiments, DNA polymerase kits are used to perform PCR amplification of the target gene fragment, which is then ligated with a vector to construct a recombinant DNA molecule. This molecule is then introduced into host cells for expression. For example, in constructing a genetically engineered bacterium for insulin production, the insulin gene is cloned and expressed in Escherichia coli to produce insulin in large quantities.
Site-Directed Mutagenesis: By designing specific primers and using DNA polymerase kits for PCR reactions, specific mutations can be introduced into the target gene. This is used to study the relationship between gene structure and function. For example, changing a certain amino acid residue in a protein and observing its impact on the protein’s activity and function.

From RNA to cDNA: In reverse transcription PCR (RT-PCR) and real-time quantitative PCR (qRT-PCR), it is first necessary to reverse transcribe RNA into cDNA, and then perform PCR amplification. The reverse transcriptase in the DNA polymerase kit can catalyze the process of synthesizing cDNA using mRNA as a template. It is used to study the expression level of genes, such as comparing the differences in gene expression levels in different tissues or under different physiological states.

Random Amplified Polymorphic DNA (RAPD): Using random primers, PCR amplification of genomic DNA is carried out under the action of DNA polymerase. By analyzing the polymorphism of the amplified products, the genetic diversity and genetic relationships of species can be studied. It is widely used in plant variety identification, genetic map construction, and other aspects.
Simple Sequence Repeat (SSR) Markers: Primers are designed for microsatellite sequences in the genome, and PCR amplification is carried out with the help of DNA polymerase kits. The genetic differences between individuals are analyzed according to the length polymorphism of the amplified fragments. It is often used in genetic breeding, population genetics research, and other fields.