HCY™ HotStart SYBR Green qPCR Master Mix

HCY™ HS-Taq DNA Polymerase is based on HCY™ Taq DNA Polymerase modified by a special chemical modification method.HCY™ HS-Taq DNA Polymerase possesses 5′-3′ DNA polymerase activity and 5′ -3′ exonuclease activity and no 3′-5′ exonuclease activity. The product carries an A base at the 3′ end and can be directly cloned with TA vector.
HCY™ HS-Taq DNA Polymerase activity is activated only after 10 minutes of pre-denaturation at 98°C, which inhibits non-specific amplification caused by non-specific annealing of the primers or primer dimerization at low temperatures. The PCR reaction with this product can be operated at room temperature, and can effectively avoid the non-specific PCR background caused by the activity of common Taq enzyme or other heat-resistant DNA polymerase during room temperature operation.

HCY™ HS-Taq DNA Polymerase is based on HCY™ Taq DNA Polymerase modified by a special chemical modification method.HCY™ HS-Taq DNA Polymerase possesses 5′-3′ DNA polymerase activity and 5′ -3′ exonuclease activity and no 3′-5′ exonuclease activity. The product carries an A base at the 3′ end and can be directly cloned with TA vector.
HCY™ HS-Taq DNA Polymerase activity is activated only after 10 minutes of pre-denaturation at 98°C, which inhibits non-specific amplification caused by non-specific annealing of the primers or primer dimerization at low temperatures. The product can be used for PCR reactions at room temperature and can effectively avoid non-specific PCR backgrounds caused by the activity of common Taq enzymes or other heat-resistant DNA polymerases at room temperature.
HCY™ HotStart SYBR Green qPCR Master Mix (2×) is suitable for the detection of Real Time PCR qualitative and quantitative reactions using SYBR Green I chimeric fluorescence method.
It contains all common components such as HS-Taq DNA Polymerase, dNTPs, Mg2+, SYBR Green I fluorescent dye, etc. It is only necessary to add template, primers and water for PCR amplification. This product adopts the optimized formulation of qPCR special Buffer, which can get a good standard curve in a wide quantification region and accurately perform quantification. This product is compatible with most manufacturers’ fluorescence quantitative PCR instruments, such as Applied Biosystems, Eppendorf, Bio-Rad and Roche.
This product contains individually packaged ROX for use in PCR instruments that require ROX as a correction dye, which is designed to minimize well-to-well variation by correcting for fluorescence fluctuations unrelated to PCR. Such differences can be caused by a variety of factors, such as pipetting errors and sample evaporation. Different fluorescence quantitative PCR instruments have different requirements for ROX.