HCY™ BloodDirect PCR Kit

The HCY™ Blood Direct PCR Kit is a kit for direct PCR amplification of whole blood samples without the need for DNA purification or sample preparation. It can be used to directly amplify fresh blood, chilled (frozen) blood, and commercially dried Whatman903® and FTA® blood stains containing conventional anticoagulants such as EDTA, heparin, and citrate. The kits contain genetically engineered BloodDirect DNA Polymerase, which is highly resistant to PCR inhibitors in whole blood samples.

Direct PCR reaction of different types of whole blood samples.

Precautions
The recommended amount of blood template to be used is 10% of the total reaction volume, i.e., 5ul of whole blood is added to a 50ul reaction system;
Aspirate anticoagulated blood, blood clots should be avoided as much as possible;
The extension time is set at 30sec/kb, if it is less than 15sec, set 15sec can be used.
After the PCR reaction is completed, it is recommended that the reaction solution be centrifuged at 4000rpm (1000×g) for 1-3min to precipitate cell debris, after which the supernatant is taken for downstream analysis.

Quality control
SDS-PAGE electrophoretic purity is greater than 98%;
Functional test: sensitivity, specificity, reproducibility of PCR;
No exogenous nuclease activity, no exogenous endonuclease and exonuclease contamination;
No expression host nucleic acid residue.
Precautions: This product is for scientific research use only, and cannot be used for medical or diagnostic procedures in humans or animals, or as food, cosmetics or household products, etc. Without written permission authorization or approval, this product may not be manufactured, licensed for sale, sold or imported or exported, or use all related patents and related trademarks of the product.
Frequently Asked Questions:

1. No amplification product or little product amount
   Check the primer design and confirm the purity and concentration of primers.
   ② Repeat the experiment to make sure that the reaction system is prepared and the reaction program is set correctly.
   ③Increase the number of cycles
   ④Increase the concentration of Mg2+ ⑤Optimize the annealing temperature
   ⑤ Optimize the annealing temperature
   ⑥Try different amounts of blood