HCYTM Salmonella（SS） Real-time PCR Detection Kit(Lyophilized Bead)

HCYTM Salmonella (SS) Fluorescence Quantitative Test Kit (Lyophilized Microspheres) is a fluorescence quantitative test for the detection of Salmonella in feces, and other samples. Specific primers and Taqman probes are designed with the conserved genes of Salmonella and amplified by fluorescence quantitative PCR instrument. Meanwhile, this kit uses the GAPDH gene of cats and dogs as the internal reference gene, which meets the requirements of indoor quality control in the whole process from sampling, sample purification to on-line testing.
This kit has the following advantages:
Designed based on the gene sequences of pathogens prevalent in various regions of the world, which fully guarantees the accuracy and validity of the test;
The primers are designed with special bases to minimize the risk of missed detection due to gene mutation;
Pre-filled lyophilized microspheres for transportation and storage at room temperature;
Easy to use, no need to prepare reagents in advance;
The lowest detection limit is 2 copis/Test;
Clinical sensitivity 100%, specificity 99.11%;
Addition of internal standard to prevent false-negative results and facilitate indoor quality control.

Quality by Design (QbD)
All Salmonella genes listed in NCBI are compared, homology comparison is performed, evolutionary tree analysis is constructed, detection targets are analyzed, and screening is performed in conjunction with literature and patents.
The test kit is designed and developed based on the above gene sequences of endemic pathogens in different regions of the world, which fully ensures the accuracy and validity of the test. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.

LoD and PCR Efficiency
Using real clinical samples, the test was performed after serial gradient dilution, and the results showed that the linear characteristics were good, and the calculated copy number could be as low as 2 copis/Test, and the calculation of the experimental data set showed that the R2=0.9988, and the amplification efficiency E=98.6%.