MDM（Iscove’s Modified Dulbecco Medium）

IMDM is Iscove’s modified DMEM medium, designed by Guilber and Iscove in 1976 for the culture of erythroid precursor cells and macrophages. IMDM medium is based on DMEM medium with selenium, HEPES, sodium pyruvate, and additional amino acids and vitamins, which is very nutrient-rich and suitable for rapid proliferation, high-density cell culture. IMDM media can be used not only to culture cells with special nutritional requirements (e.g. mouse B-lymphocytes, LPS-stimulated B-cells, bone marrow hematopoietic cells, T-lymphocytes, and a variety of hybridoma cells), but also as a basal fluid for a number of unique serum-free culture media.
For a wide range of cell culture applications, we offer IMDM modified media in a variety of components.

L-glutamine (Glutamine) is an essential nutrient in cell culture, but it is unstable in solution and will degrade spontaneously. L-glutamine-free media can be used to arbitrarily adjust the dosage of L-glutamine according to the research needs, and the addition of fresh L-glutamine or its substitutes at the time of use is more conducive to the growth of cells.
L-Alanyl-L-glutamine (Ala-Glu), also known as alanyl-glutamine and alanine-glutamyl dipeptide, is an advanced cell culture additive that can be used as a direct replacement for L-glutamine in cell culture media.L-glutamine is an essential nutrient in cell culture, but it is unstable in solution and will L-glutamine is an essential nutrient for cell culture, but it is unstable in solution and will spontaneously degrade to ammonia and pyroglutamic acid, of which ammonia is harmful to cells; whereas L-alanyl-L-glutamine is very stable in aqueous solution and will not spontaneously degrade. The mechanism of cellular utilization is: in cell culture, cells will gradually release a peptidase into the culture medium, which hydrolyzes L-alanyl-L-glutamine into L-alanine and L-glutamine, and then the cells will take up these two hydrolysis products and utilize them. The process of cellular utilization of L-alanyl-L-glutamine is similar to the flow-addition culture strategy, in which low concentration levels of L-glutamine are continuously added to the culture medium, thereby increasing the utilization of L-glutamine without generating excess ammonia, which is more conducive to cellular growth.L-alanyl-L-glutamine can be substituted for an equimolar L-glutamine, which is suitable for all cells with little need for acclimatization, and can extend the time of cell culture. And it can prolong the cell culture time and reduce the number of passages, which saves time and money. Activity decreases more slowly than in cells cultured in medium supplemented with L-glutamine. The reason for the slightly longer delay is the time required for the release of peptidase and the digestion of dipeptides.
Phenol red is used as a pH indicator in the medium to continuously monitor the pH of the culture medium. At low pH values phenol red gives the culture medium a yellow color, while at higher pH values it gives the culture medium a purple color, with a red color at pH values of 7.2-7.4, which is best suited for cell culture. However, phenol red also has some disadvantages, studies have shown that phenol red can mimic the effects of steroid hormones (especially estrogen), so when used to estrogen-sensitive cells (such as breast tissue), it is best to use a medium that does not contain phenol red. Phenol red can interfere with detection during flow cytometric analysis. In addition, the presence of phenol red in some serum-free media formulations can interfere with sodium-potassium balance.
Penicillin-streptomycin mixture, often referred to as “double antibiotic”, is the most commonly used antibiotic to prevent microbial contamination in in vitro cultures. Penicillin interferes with bacterial cell wall synthesis and is particularly effective against Gram-positive bacteria, while streptomycin binds to the 30S subunit of the bacterial ribosome and inhibits bacterial protein synthesis, which is particularly effective against Gram-negative and Gram-positive bacteria. Gram-negative and Gram-positive bacteria, but particularly effective against Gram-negative bacteria. The combination of penicillin and streptomycin prevents most bacterial contamination.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, but does not contain proteins, lipids or any growth factors, so this product should be used with serum or serum-free additives.