Adenosine Triphosphate (ATP)

Adenosine Triphosphate (ATP) is a direct source of energy for a variety of life activities and is found in plant and animal cells, bacteria and food residues.ATP fluorescence assay is a rapid detection technique developed based on the glow of fireflies. That is, in the presence of ATP, recombinant luciferase can catalyze the oxidation of the substrate D-luciferin and emit fluorescence. The number of photons is linearly related to the amount of ATP within a certain range in the presence of an excess of substrates other than ATP. Therefore, the intensity of the fluorescence signal can be measured to know the degree of contamination of the target to be measured by bacteria, food residues, etc.

The sensitivity of this reagent is 5×10-17mol ATP
Convenient and quick: integrated liquid stabilized detection swab, simple detection operation procedure;
Good reproducibility: RLU output is stable and reliable, with high reproducibility;
High sensitivity: can detect up to 5×10-17mol ATP.
Domestic ATP fluorescence detection swab use method:

Leave the swab in refrigerated or frozen condition at room temperature for about 10 minutes.
Squeeze the illustration 2 with one hand and pull it out by gently rotating the sealing tube with the other hand (do not pull the sealing tube out directly!) .
Wipe the surface of the object evenly with a cotton tip in a zigzag shape of about 10*10cm2, or take it out quickly after immersing it in the liquid to be tested. When finished, put the sealing tube on.
Fracture the sealing rod and squeeze the illustration 1 so that the solution contained therein flows down the inlet hole, all the way to the cotton tip, and shake the bottom of the swab for about 2 seconds.
Quickly place the swab into the ATP fluorescence detector, press the OK button and read the reading after 15 or 10 seconds.

Domestic ATP fluorescence detection swab preservation and expiration date:
-10 ℃ to 20 ℃ storage, try to avoid repeated freezing and thawing of reagents; short time storage, can be stored at 4 ℃, pay attention to avoid light, sealed storage; validity period of 12 months. Please do not use the reagents that have passed the shelf life.
Domestic ATP fluorescence detection swab (ATP sampler) is suitable for all kinds of domestic and imported fluorescence detector. When used, specimens are collected with pre-moistened ATP swabs. ATP swabs are moistened with buffer, which helps to extract biomass (ATP) from dry or wet surfaces. The swab contains a reagent that cleaves the cell membrane, releasing intracellular ATP, which reacts with specific enzymes contained in the reagent to produce light, which is then detected by a fluorometer, and the number of microorganisms is directly proportional to the luminescence value.
ATP fluorescence test swabs using a specialized type of sample collection and reaction of the integrated rapid detection device, supporting the use of ATP fluorescence detector.
The swabs use the world’s best liquid stabilized reagents, which are more convenient and easy to use, providing more accurate, longer luminescence signal and better reproducibility of the test results.
Can be kept refrigerated for long periods of time.
Detects dry surfaces and liquid samples.
Results in 15 seconds.
Suitable for all kinds of domestic and imported ATP fluorescence testers.
The price of ATP fluorescence swabs is only half of imported ATP swabs.
Good reproducibility and high reliability of the reaction results, environmentally friendly design.
Domestic ATP Fluorescence Swab

Ultrasnap ATP integrated ATP detection swabs, unique integrated liquid reagent, collection reaction in one, extremely easy to operate, shelf life of 12 months (2-8 ℃); room temperature 6 weeks (25 ℃ the following), no need to freeze Preservation, than the traditional dry-cooled luciferase has a great advantage, good accuracy and reproducibility, stability, not easy to collect the impact of ambient temperature, swabs pre-moistened, to reduce the degree of contamination of the operating chain. The swabs are pre-moistened to minimize the contamination during the operation.

Precautions:

This reagent needs to be matched with an ATP fluorescence detector.
ATP Quickswab contains luciferase, which is gradually inactivated by repeated freezing and thawing. In order to achieve better results, the number of freezing and thawing should not be more than 3 times, and should be kept away from light.
For good results, the number of freezing and thawing should not be more than 3 times, and should be kept away from light.
Wear disposable gloves to avoid contamination by exogenous ATP.
Do not touch the swabs or swabs during the sampling process, and make sure that the swabs are in direct contact with the surface of the object to be measured.
After the reaction of the sample and solution in the swab, it needs to be placed in the fluorometer and read within 60 seconds.
The smear area for standard operation is 10 x 10 cm square. For irregular object surfaces, it is important to ensure that each test for each control point is
A continuous and consistent method is used. The control points should be standardized to take into account the particular structure of different tables, such as table smoothness, instrument seams, recessed areas, cutlery, and other items.
seams, instrument seams, recessed areas, and the presence of cracks in utensils (which tend to harbor dirt).
This instrument measures the cleanliness of surfaces with low resolution to the naked eye. Therefore, if the control point to be measured has dirt visible to the naked eye, or if the head of the swab is visibly changed after application, the instrument will not be able to detect it.
If there is dirt visible to the naked eye at the control point to be measured, or if the head of the swab becomes visibly darker after application, stop subsequent operations to avoid wasting the swab.
If there is excess liquid on the surface of the object to be inspected, wait until the liquid has dried slightly before performing the test to avoid diluting the reagent. (No special
drying)
If you need to test the liquid, you can use the sampler to suck and add two drops of the sample in the test tube, put the test tube and shake, and then mix with the luminous reagent.
(Do not swab the liquid directly.)