Portable Real-Time Fluorescence Quantitative PCR – Ushering in a New Era of On-Site Testing”

With the continuous progress of science and technology, portable real-time fluorescence quantitative PCR has brought revolutionary changes to on-site testing, and its application value has become increasingly prominent in various fields.

Portable real-time fluorescence quantitative PCR has a wide range of applications in food safety testing. It can quickly detect pathogenic microorganisms in food, such as Escherichia coli and Salmonella. At the site of food production and processing, it can conduct timely detection on raw materials, semi-finished products, and finished products. Once excessive microorganisms are found, immediate measures can be taken to deal with them, preventing problem foods from entering the market and ensuring the food safety of consumers. In the detection of agricultural products, it can also detect transgenic components, which is of great significance for strictly controlling the market circulation of transgenic foods. For example, in some large wholesale markets for agricultural products, staff can use portable devices to quickly screen vegetables, fruits, and other agricultural products to ensure that they meet relevant standards.

This device has numerous advantages. Its portability makes testing no longer limited to the laboratory environment. Whether in the workshop of a food processing factory or at the base of agricultural product cultivation, testing can be carried out anytime and anywhere. Its rapidity has greatly improved the efficiency of food testing. Testing projects that previously took several days to complete can now obtain results within a few hours, greatly shortening the time to market for food. High sensitivity and specificity ensure the reliability of test results. It can accurately detect trace amounts of pathogenic microorganisms and transgenic components, providing a solid guarantee for food safety. Meanwhile, it is easy to operate. Staff who have received simple training can operate it, reducing the dependence on professional technicians and increasing the popularity of testing.

When using portable real-time fluorescence quantitative PCR, the following points should be noted. Firstly, during the sample processing process, cross-contamination of samples should be avoided to ensure the independence and accuracy of each sample. Secondly, the use environment of the instrument should be kept stable to avoid the influence of environmental factors such as temperature and humidity on the test results. Thirdly, the performance of the instrument should be regularly verified to ensure its accuracy and reliability. For the interpretation of test results, comprehensive judgment should be made in combination with the actual situation, and one should not rely solely on a single test data.

In summary, portable real-time fluorescence quantitative PCR plays an important role in the field of food safety testing with its unique advantages, providing a powerful means to ensure the safety of people’s dining tables and ushering in a new era of on-site rapid testing.