A cell preservation solution is a special solution used to preserve cells. It mainly has the following functions: Maintaining cell activity: providing a suitable environment to keep cells in a better state of survival for a certain period of time. Preventing cell damage: reducing the damage of physical, chemical and other factors to which the cells are subjected during the preservation process. Facilitate transportation and storage: enable cells to be transported and stored under different conditions without affecting their subsequent use. Different types of cells may require the use of specific cell preservation solutions to meet their preservation needs.
The main components of cell preservation solutions usually include the following: Buffer system: such as phosphate buffer, etc., to maintain a certain pH value and stabilize the cellular environment. Nutrients: such as glucose, amino acids, etc., to provide energy and nutrition for cells. Protective agents: such as dimethyl sulfoxide (DMSO), etc., to reduce cell damage. Anticoagulants: e.g. ethylenediaminetetraacetic acid (EDTA) to prevent blood clotting. Antimicrobials: e.g. gentamicin, etc. to prevent microbial contamination.
The pH value of cell preservation solution has an important influence on the effect of cell preservation, which is mainly reflected in the following aspects: Maintaining normal cell metabolism: suitable pH value helps to maintain the activity of intracellular enzymes and normal metabolic processes, ensuring the physiological function of cells. Preventing cell damage: An overly acidic or alkaline environment may lead to damage to the cell structure and affect cell survival. Affecting cell stability: Inappropriate pH may make cells more susceptible to external factors, reducing the stability of cell preservation.
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