Cell culture medium powder

Usage Methods

1. Preparation Work
   • Ultra – pure water or deionized water needs to be prepared, because impurities in water may affect the quality of the culture medium. At the same time, suitable containers, such as glass beakers or plastic containers, need to be prepared. The containers need to be strictly cleaned and sterilized to prevent contamination by impurities and microorganisms.
2. Dissolution Process
   • According to the ratio of powder to water specified in the product manual, slowly add the cell culture medium powder to the water. Generally, add it while stirring to ensure that the powder is evenly dispersed in the water and prevent lumps from forming. Stirring is usually carried out with a magnetic stirrer or a glass rod, and the stirring speed is moderate to avoid generating too many bubbles.
3. Adding Other Components
   • According to the specific requirements of cell culture, add other components such as serum, antibiotics, and growth factors to the dissolved culture medium. For example, for most mammalian cell cultures, fetal bovine serum (FBS) is usually added, and the addition amount is generally between 5% and 20%, to provide additional nutrients and growth factors required for cell growth.
4. pH Adjustment and Filtration Sterilization
   • Use pH test paper or a pH meter to detect the pH value of the culture medium and adjust it with acid (such as hydrochloric acid) or alkali (such as sodium hydroxide) as needed to make it reach the pH range suitable for cell growth (usually between 7.2 and 7.4). Finally, filter the prepared culture medium through a sterile filter membrane of 0.22 μm to remove possible microorganisms and ensure the sterility of the culture medium before it can be used for cell culture.

Application Fields

1. Basic Cell Biology Research
   • In the research of cell structure and function, researchers can use cell culture medium powders with different formulas to prepare culture media for culturing cells. For example, when studying mitochondrial function, a suitable culture medium is precisely prepared to maintain the normal growth of cells, and then the morphological, distributional, and functional changes of mitochondria in cells can be observed in – depth.
2. Biomedical Research and Development
   • In drug screening and toxicity testing, cells are cultured in culture media prepared from cell culture medium powders. For example, in the research and development of anti – cancer drugs, tumor cells are inoculated in a culture medium prepared from powder and cultured, and then the drugs to be tested are added. The anti – cancer efficacy and toxicity of the drugs are evaluated by observing the growth, survival, and morphological changes of the cells.
3. Biological Product Production
   • In the production process of monoclonal antibodies and recombinant proteins, suitable cell culture medium powders are crucial. By optimizing the formula of the culture medium, the growth rate of production cells (such as hybridoma cells, engineered cells, etc.) and the product expression level can be improved, thereby improving the production efficiency and quality of biological products.