Cell-Free DNA Blood Collection Tube

Features

Room temperature preservation: Contains unique anticoagulation and preservation reagents, which can be stably transported and stored for 7 days or more at room temperature.

The blood samples were collected into HCY BCT and conventional EDTA BCT. After being placedat room temperature for 5 days, the fragment size and peak pattern of free DNA were detected by a bioanalyzer. It can be seen that conventional EDTA BCT cannot prevent cfDNA degradation.

Stable storage: It can stabilize nucleated cells and prevent the release of cell genomic DNA from contaminating target free DNA; inhibit nuclease degradation of free DNA to ensure sample quality.

Personalized design: Double-coded label design, easy to identify, reduce sample processing errors;

clear blood sampling volume identification, easy to observe; there are various material and size for

choice, suitable for different scenarios.

Wide range of applications: Used for NIPT, tumor liquid biopsy, blood pathogenic microorganism detection, genetic disease detection; third-party medical laboratory, hospital laboratory and obstetrics, etc.

• Preventing Cell Rupture and DNA Release
  • Containing Special Anticoagulants: Special anticoagulants such as EDTA and citrate are generally added. These anticoagulants can effectively prevent blood clotting and stop the rupture of cells caused by factors such as platelet activation during the blood clotting process. This avoids the release of intracellular DNA into the plasma, which could affect the purity of cfDNA and the accuracy of test results.
  • Optimized Physical Properties: The material and inner wall treatment of the collection tube are specially designed and have good biocompatibility. This can reduce the friction between blood cells and the tube wall, and lower the risk of mechanical damage and rupture of cells. It keeps the blood cells as intact as possible, ensuring that the collected cfDNA is mainly the cell-free DNA naturally released into the blood through physiological processes such as apoptosis or necrosis of cells.
• Stable Protection of cfDNA
  • Inhibiting Nuclease Activity: There are various nucleases in the blood, which can degrade cfDNA. Nucleic acid enzyme inhibitors are usually added to cfDNA collection tubes. These inhibitors can rapidly inhibit the activity of nucleases, preventing the degradation of cfDNA. Thus, it ensures that the integrity and quantity of cfDNA remain unaffected for a certain period after sample collection, providing a stable sample for subsequent detection and analysis.
  • Maintaining an Appropriate pH Environment: The buffer system inside the collection tube can maintain the blood within an appropriate pH range, avoiding the impact of drastic changes in pH on the structure and stability of cfDNA. This ensures that cfDNA can maintain its biological activity and integrity in the in vitro environment.
• Facilitating the Separation of Cells and Plasma
  • Assistance from Components in the Tube: Some cfDNA collection tubes contain special components such as separation gel. During the centrifugation of blood, the separation gel will form a clear interface between the cells and the plasma, effectively separating the cells from the plasma. This prevents the cells from coming into contact with the cfDNA in the plasma again, avoiding the interference of intracellular substances with the detection of cfDNA in the plasma, and improving the extraction efficiency of cfDNA and the accuracy of detection.
  • Design Conducive to Stratification: The shape and size design of the collection tube also help to achieve good stratification of cells and plasma during centrifugation, making it convenient to extract and analyze the cfDNA in the plasma.
• Convenient for Transportation and Storage
  • Stable at Room Temperature: Some advanced cfDNA collection tubes can ensure the stability of cfDNA under room temperature conditions. This allows the sample not to require immediate low-temperature storage and transportation after collection, greatly improving the convenience and flexibility of sample transportation, reducing the cost and difficulty of cold chain transportation, and facilitating the smooth transportation of samples from the collection site to the testing laboratory for analysis.
  • Long-term Storage Performance: Under appropriate storage conditions, cfDNA collection tubes can ensure the stability of cfDNA within a certain period of time, meeting the needs of some retrospective studies or multiple tests that require long-term sample storage, providing convenience for clinical diagnosis and scientific research work.
• Standardization and Versatility
  • Uniform Specifications: cfDNA collection tubes usually have standardized specifications and designs, which are convenient for operation and result comparison between different laboratories and medical institutions. Whether in large clinical testing centers or small scientific research laboratories, the same specification of collection tubes can be used for sample collection, ensuring the consistency of experimental operations and the comparability of results.
  • Compatibility with Various Detection Technologies: Their design can be adapted to a variety of common cfDNA detection technologies, such as quantitative PCR, next-generation sequencing (NGS), digital PCR, etc. Regardless of which detection method is used, the samples collected and preserved by cfDNA collection tubes can provide a reliable basis for accurate detection, meeting different clinical and scientific research needs.