Feline Immunodefiency Virus Antibody Test Kit (FIV-Ab)

Feline HIV Antibody Kit Product Description
Feline HIV Antibody Kit is used for the detection of feline HIV antibody in whole blood or serum of cats, for screening and auxiliary diagnosis of feline AIDS infection.
Feline Immunodeficiency Disease (FID) is a viral infectious disease caused by Feline Immunodeficiency Virus (FIV) infection, characterised by immune deficiency, inflammation of the respiratory and digestive systems, dysfunction of the immune system and the nervous system, and susceptibility to secondary infections. It is also called feline AIDS because the biological characteristics of the pathogen and the symptoms of infected cats are similar to those of human AIDS.FIV is a lentivirus of the family Retroviridae, the only member of the feline lentivirus group. The virus particles are spherical or oval, with a rod-shaped or conical capsid, and are released by budding from the cell membrane of the infected cell, and the viral genome is a single-stranded, positive-stranded linear RNA dimer.FIV infections are prevalent worldwide, with a high prevalence in middle-aged and old-aged cats, and are transmitted among cats mainly through bites, and are strictly host-specific, as are other lentiviruses.

Reaction Principle】
Cat HIV Antibody Kit uses rapid immunochromatographic technique to detect cat HIV antibody in cat whole blood or serum. After the diluted sample is dropped into the spiking well, if FIV antibody is present in the sample, it will specifically bind to the colloidal gold-labelled recombinant protein, forming a complex that moves together along the chromatographic membrane and is captured by the FIV antigen encapsulated on the membrane, displaying a burgundy line on the detection line (T). If there is no FIV antibody in the sample, no visible line will form at the T position. This C line should be coloured regardless of negative or positive results, otherwise the result is judged invalid.

How to use the Cat HIV Antibody Test Kit
[Sample Requirements
This kit is only suitable for testing cat whole blood or serum samples.
The test sample is collected fresh and put into the dilution solution immediately, and the diluted sample should be tested as soon as possible within 1 hour.
If the sample can not be detected in time, it can be stored at 2-8℃ for 24 hours and -20℃ for 1 month.
[Detection Method
Before use, the test card and dilution solution should be restored to room temperature and the instructions of the kit should be read carefully.
Use whole blood samples from cats, let it stand for 30 minutes to precipitate or separate the serum by centrifugation for spare.
Tear open the foil pouch of the test card, take out the test card and place it on a flat, clean countertop.
Using a pipette, add 2 drops of whole blood or serum to the sample well, then add 1 drop of diluent, start the timer and wait for the results.
Wait 10-15 minutes at room temperature for the test result to be read; results after 20 minutes are invalid.
Note: The reading time is performed at room temperature 15-30°C. If the temperature is lower than 15°C, the reading time should be extended appropriately; if the temperature is higher than 30°C, the reading time should be shortened appropriately.

Precautions]
The reagents provided with this product are for this test only, please read the instructions carefully before the test.
Expired, broken or contaminated aluminium foil pouch should not be used.
The product should be restored to room temperature before use and used within 1 hour after opening to avoid moisture.
Avoid contact with the spiking hole on the test card and the chromatographic membrane at the test window during use of the product.
The samples tested may be potentially infectious and the waste after the test should be regarded as contaminants and disposed of properly in accordance with relevant local regulations.