Infectious Canine Hepatitis Antigen Test Kit (ICH-Ag)

This kit is used to detect infectious canine hepatitis antigens in canine feces, oral, eye and nasal secretions for screening and auxiliary diagnosis of infectious canine hepatitis infection, etc.
Infectious canine hepatitis (ICH) is an acute, septic infectious disease caused by the infectious canine hepatitis virus, characterized by hemorrhagic gastroenteritis, circulatory disorders, necrosis of the central lobules of the liver, and the appearance of intranuclear inclusions in the liver parenchymal and endothelial cells. The infectious canine hepatitis virus belongs to the Adenoviridae family in classification and is a member of the Mammalian Adenovirus genus, a double-stranded DNA virus. Sick dogs and virus-carrying dogs are both the main sources of infection, and their excretions and secretions will carry a large amount of virus. Recovered dogs can excrete the virus for a long time through urine. Healthy dogs are transmitted to susceptible animals through the digestive tract by directly contacting the excretions and secretions of sick dogs or utensils carrying the virus. The morbidity and mortality rates of puppies are relatively high.
[Reaction Principle] This product adopts rapid immunochromatographic technology to detect infectious canine hepatitis antigens in canine feces, oral, eye and nasal secretions. After the diluted sample is dropped into the sample adding hole, if there is an ICH antigen in the sample, it will specifically bind with the colloidal gold-labeled recombinant protein to form a complex and move along the chromatographic membrane together, and be captured by the ICH antibody coated on the membrane, showing a wine-red line on the detection line (T). If there is no ICH antigen in the sample, no visible line will be formed at the T position. Regardless of the negative or positive result, the C line should show color, otherwise it will be judged as an invalid result.

【Sample Requirements】

This kit is only applicable to the detection of canine feces and oral, eye and nasal secretion samples.
Collect fresh samples for the detection samples and immediately put them in the diluent. The diluted samples should be tested as soon as possible within 1 hour.
If the samples cannot be tested in time, they can be stored for 24 hours at 2-8°C and for 1 month at -20°C.

1. Use a disposable sampler to collect canine feces and oral, eye and nasal secretion samples, put them in the diluent and stir well, take out the sampler, tighten the lid, invert and mix well, and let it stand for 1 min.
2. Tear open the aluminum foil bag of the test card, take out the test card, and place it on a flat and clean tabletop.
3. Use a straw to absorb 3 drops (about 80 μL) of the diluted sample into the sample addition hole of the test card and wait for the test result.
4. Wait for 10-15 min at room temperature to interpret the test result, and the test result after more than 20 min is invalid.
   Note: The above interpretation time is carried out at room temperature of 15-30°C. If the temperature is lower than 15°C, the interpretation time should be appropriately extended; if the temperature is higher than 30°C, the interpretation time should be appropriately shortened.