Nucleic Acid Extraction Kit

Uses of nucleic acid extraction or purification reagents

It is used for nucleic acid extraction, enrichment and purification steps. Its processed product is used for clinical in vitro testing use.
Usage of nucleic acid extraction and purification reagents:

I. Before using nucleic acid extraction reagent

Transfer the proteinase k solvent into the lyophilized powder containing proteinase k and mix well.
Add 18 ml and 42 ml of anhydrous ethanol to CY3 and CY4 of CY-F006-10 (50preps-cells) and CY-F006-20 (50preps-saliva), respectively, and mix well.
Add 36 ml, 84 ml of anhydrous ethanol to CY3 and CY4 of CY-F006-11 (100preps-cells) and CY-F006-21 (100preps-saliva), respectively, and mix well.
Second, swab extraction step:

Swab dry extraction add 0.6ml of CY1 liquid and 10ul protease for mixing, place in 65℃ air incubator for 30 minutes (or wet extraction: sample centrifuge tube containing swab plus preservation solution centrifuged at 12000rpm for 1 minute, retain the precipitate and remove the supernatant. Add 0.6ml CY1 liquid, 10ul proteinase k, mix well and place in air incubator at 65 degrees C for 30 minutes)
Remove swab and centrifuge at 12000rpm for 1 minute
Remove all the supernatant to a new centrifuge tube and perform the experiment
Add 0.25ml CY2 liquid and 10ul magnetic beads (shake well before use), mix well for 12min, place on a magnetic stand for 30s, aspirate off the liquid
Add 0.6ml of CY3 liquid, mix for 3min, place on magnetic stand for 30s, aspirate the liquid.
Add 0.6ml of CY4 liquid, mix for 3min, place on a magnetic rack for 30s, absorb the liquid.
Repeat steps 2~6
Dry at room temperature for 10-20 minutes, add 50ul of CY5 liquid to elute, mix well, place on a magnetic rack for 30s, transfer liquid to a new centrifuge tube
Measure OD

III. Saliva Extraction Procedure

Centrifuge saliva plus preservation mixture at 12000rpm for 1 minute.
Retain the precipitate and remove the supernatant
Add 0.6ml of CY1 liquid and 10ul of proteinase k, mix well and place in air incubator at 65 degrees Celsius for 30 minutes.
Centrifuge at 12000rpm for 1 minute, remove all the supernatant to a new centrifuge tube, add 10ul magnetic beads and 0.25ml CY2, mix well for 12 minutes, place on a magnetic stand for 30s and aspirate the liquid.
Add 0.6ml CY3 liquid, mix for 3 minutes, place on a magnetic stand for 30s and aspirate the liquid.
Add 0.6 ml of CY4 liquid, mix for 3 minutes, place on a magnetic stand for 30s and aspirate the liquid.
Repeat step 6
Dry at room temperature for 10~20 minutes, add 50ul of CY5 liquid to elute, mix well, place on a magnetic rack for 30s, transfer liquid to a new centrifuge tube.
Measure OD
Note: If you need to remove RNA, you can prepare your own RNaseA10mg/ml: solvent (10mM sodium acetate: pH5.0), boil for 15min to adjust pH7.5 with Tris-Hcl, store at -20 degree Celsius.