Minimum Essential Medium (MEM

Minimum Essential Medium (MEM) is one of the more commonly used cell culture media. It was developed by Harry Eagle based on his earlier formulation of Basic Eagle Medium (BME). Many other modifications of MEM have been made since then, including Glasgow’s MEM, MEM alpha, DMEM, and Temin’s modified media. 1080, MCF-7, fibroblasts, and primary rat astrocytes, etc. MEM cell culture medium contains no proteins, lipids, or growth factors. Therefore, supplemental nutrients are required, usually 10% fetal bovine serum (FBS).MEM contains Earle’s Balanced Salts for CO2 incubators or Hanks’ Balanced Salts for CO2-free incubators.
For a wide range of cell culture applications, we offer modified MEM media in a variety of components, as well as MEM media with customizable compositions.

This MEM dry powder medium is prepared with sodium bicarbonate, pH adjustment and filtration. The detailed preparation protocol is as follows:
Add 950 mL of distilled water to a mixing vessel as close to the final volume as possible.
Add dry media to room temperature (15°C to 30°C) water and stir gently. Do not heat the water.
Rinse the inside of the package to remove any residual powder.
Add 50 mL of 75 g/L NaHCO3 solution to the medium.
Adjust pH to 0.2 to 0.3 units below desired final working pH by slowly adding and stirring in 1 N NaOH or 1 N HCl. The pH may rise 0.1 to 0.3 units after filtration. The recommended working pH is 7.0 to 7.4.
Adjust final volume using distilled water.
Immediately process the medium through a 0.2 μm filter membrane into a sterile container using the Positive Pressure Filtration System.