M199 Dry Powder Medium

M199 Dry Powder Medium

M199, known as Medium 199, was designed by Morgan et al. in 1950, initially for the nutritional study of chicken embryo fibroblasts, and is now widely used in the culture of a variety of animal cells, including some non-mammalian cells.M199 is particularly suitable for the culture of non-transformed cells, and is also commonly used in virology, vaccine production, and the in vitro culture of primary explants of rat pancreatic epithelium and mouse lens tissue. and primary explants of mouse lens tissue in vitro. M199 contains a unique composition compared to other basal media, including adenine, adenosine, hypoxanthine, thymine, and other vitamins.M199 is available in two balanced salt compositions, Earle’s Salt for use in CO2 environments, and Hank’s Salt for use in non-CO2 environments.M199 is free of proteins, lipids, and lipids. The medium contains no proteins, lipids, or growth factors.199 The medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiologic pH.
For a wide range of cell culture applications, we offer M199 Modified Medium in a variety of components, as well as M199 Medium with customizable compositions.

This M199 Dry Powder Cell Culture Medium needs to be supplemented with sodium bicarbonate, pH adjusted and filtered during preparation. The detailed preparation protocol is as follows
Add 950 mL of distilled water to a mixing vessel as close to the final volume as possible.
Add dry powdered medium to room temperature (15°C to 30°C) water and stir gently. Do not heat the water.
Rinse the inside of the package to remove any residual powder.
Add 32 mL of 75 g/L NaHCO3 solution to the medium.
Adjust pH to 0.2 to 0.3 units below desired final working pH by slowly adding and stirring in 1 N NaOH or 1 N HCl. The pH may rise 0.1 to 0.3 units after filtration. The recommended working pH is 7.0 to 7.4.
Adjust final volume using distilled water.
Immediately process the medium through a 0.2 μm filter membrane into a sterile container using the Positive Pressure Filtration System.