Real-time fluorescent quantitative PCR technology has the advantages of high sensitivity, strong specificity, and accurate quantification. It is widely used in multiple fields. Here are some main application fields:

Pathogen detection: Can quickly detect pathogens such as viruses, bacteria, and fungi, such as the new coronavirus, influenza virus, hepatitis B virus, Mycobacterium tuberculosis, etc. It is used for early diagnosis of diseases, epidemic monitoring and prevention and control.
Disease diagnosis and typing: Detect mutations, amplifications or deletions of tumor-related genes to assist in the diagnosis, typing and prognosis judgment of tumors; diagnose and type genetic diseases caused by gene mutations.
Drug efficacy monitoring: By detecting changes in the expression of specific genes or pathogen load before and after treatment, evaluate the effect of drug treatment and provide a basis for adjusting the treatment plan.
Minimal residual disease monitoring: After tumor treatment, detect a small amount of residual tumor cells in the body to help detect the risk of recurrence in time and take corresponding treatment measures.

Gene expression analysis: Detect the expression level of specific genes in different tissues, cell types, developmental stages or experimental conditions, and study the function and regulatory mechanism of genes.
Gene copy number analysis: Determine the copy number changes of genes in the genome. For example, detecting gene amplification or deletion is of great significance for studying gene evolution, genetic diversity and its association with diseases.
Molecular marker screening: In research such as genetic map construction, gene localization and molecular marker-assisted selection, molecular markers closely linked to target traits can be screened out for genetic breeding and species evolution research.

Microbial detection: Quickly detect pathogenic bacteria in food, such as Escherichia coli, Salmonella, Listeria, Staphylococcus aureus, etc., to ensure the microbial safety of food.
Detection of genetically modified ingredients: Detect genetically modified ingredients in food to ensure consumers’ right to know and choice and meet the needs of food safety supervision.

Microbial monitoring: Analyze the structure and quantity of microbial communities in environmental samples, such as detecting bacteria, viruses, fungi and other microorganisms in water, soil and air, and evaluate the degree of environmental pollution and the health status of ecosystems.
Biodiversity research: Through quantitative analysis of DNA or RNA in the environment, understand the biodiversity in different ecological environments and provide a basis for ecological environment protection and restoration.

Screening of inbound personnel: Detect whether inbound personnel carry infectious disease pathogens such as malaria, dengue fever, Ebola, etc., to prevent cross-border transmission of infectious diseases.
Cargo detection: Quickly detect pathogens and harmful organisms in imported animals and animal products, plants and plant products to ensure the biological safety of national borders.