HCYTM Feline leukemia virus（FeLV） Real-time PCR Detection Kit (Lyophilized Bead)

HCYTM Feline Leukemia Virus (FeLV) Fluorescence Quantitative Test Kit (Lyophilized Microspheres) is a fluorescence quantitative assay for the detection of feline leukemia virus in blood, saliva and other samples. Specific primers and Taqman probes are designed with the conserved genes of feline leukemia virus and amplified by fluorescent quantitative PCR instrument. Meanwhile, this kit uses the cat RPS7 gene as the internal reference gene, which meets the requirements of indoor quality control in the whole process from sampling, sample purification to on-line testing.
This kit has the following advantages:
Designed based on the gene sequences of pathogens prevalent in various regions of the world, which fully ensures the accuracy and validity of the test;
The primers are designed with special bases to minimize the risk of missed detection due to gene mutation;
Pre-filled lyophilized microspheres for transportation and storage at room temperature;
Easy to use, no need to prepare reagents in advance;
The minimum detection limit is 5 copies/Test;
Clinical sensitivity 100%, specificity 99.11%;
Addition of internal standard to prevent false-negative results and facilitate indoor quality control.

Quality by Design (QbD)
Feline Leukemia Virus (MW839565 Reference Sequence ), gene matching with all feline leukemia virus genes included in NCBI, homology comparison, construction of evolutionary tree analysis, analysis of detection targets.
This test kit is designed and developed based on the above gene sequences of pathogens prevalent in different regions of the world, which fully ensures the accuracy and validity of the test. Meanwhile, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.

LoD and PCR Efficiency
Using real clinical samples, the test was performed after successive gradient dilution, and the results showed good linear characteristics, and the calculated copy number could reach as low as 5.5 copies/Test, the calculation of the experimental data set can be obtained as R20.9959, and the amplification efficiency E=96.9%.