HCYTMTritrichomonas foetus(TRF) Real-time PCR Detection Kit(Lyophilized Bead)

HCYTM Trichomonas Fetus (TFo) Fluorescence Quantitative Test Kit (Lyophilized Microspheres) is a fluorescence quantitative test for the detection of Trichomonas Fetus in samples of ocular and nasal secretions, feces, ascites and viscera. Specific primers and Taqman probe are designed with the conserved gene of Trichomonas foetus and amplified by fluorescence quantitative PCR. Meanwhile, this kit uses the GADPH gene of dogs and cats as the internal reference gene, which meets the requirements of indoor quality control in the whole process from sampling, sample purification to on-line testing.
This kit has the following advantages:
Designed based on the gene sequences of pathogens prevalent in various regions of the world, which fully guarantees the accuracy and validity of the test;
The primers are designed with special bases to minimize the risk of missed detection due to gene mutation;
Pre-filled lyophilized microspheres for transportation and storage at room temperature;
Easy to use, no need to prepare reagents in advance;
The minimum detection limit is 8 copies/Test;
Clinical sensitivity 100%, specificity 99.11%;
Addition of internal standard to prevent false-negative results and facilitate in-house quality control.

Quality by Design (QbD)
The reference sequence of Trichinella foetus (TFo) strain (MT362985.1) was compared with 100 items included in China, Spain, Iran and the United States combined, and an evolutionary tree was constructed to confirm the existence of multiple subspecies of TFo homology, and the Chinese region (MT362985.1) was compared for homology, and an evolutionary tree was constructed for analysis.
This test kit is designed and developed based on the above gene sequences of endemic pathogens in different regions of the world, which fully ensures the accuracy and validity of the test. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.