Infectious Canine Hepatitis Antigen Test Kit (ICH-Ag)

[Intended Use
This kit detects infectious hepatitis antigen in canine feces, oral, ocular and nasal secretions, and is used for screening of canine infectious hepatitis infection, auxiliary diagnosis, etc.
It is used for screening and diagnosis of canine infectious hepatitis.
Infectious Canine Hepatitis (ICH) is an acute infectious disease caused by canine infectious hepatitis virus,
Infectious Canine Hepatitis (ICH) is an acute, septic infectious disease caused by the canine infectious hepatitis virus, characterized by hemorrhagic gastroenteritis, circulatory disorders, central necrosis of the hepatic lobules, and the appearance of nuclear inclusion bodies in the liver parenchyma and endothelial cells.
characterized by hemorrhagic gastritis, circulatory disorders, central necrosis of the liver lobules, and the presence of nuclear inclusion bodies in the liver parenchyma and endothelial cells. Canine infectious hepatitis virus is classified as a member of the family Adenoviridae, a genus of mammalian adenoviruses, and a double stranded
DNA virus. Both sick and carrier dogs are the main source of infection, carrying large amounts of virus in their feces and secretions.
Convalescent dogs may pass the virus in urine over a long period of time. Healthy dogs are infected to susceptible animals through direct contact with excretions and secretions of sick dogs or virus-carrying utensils, and through the digestive tract.
Healthy dogs can be infected to susceptible animals through direct contact with the excreta of sick dogs or utensils with virus, through the digestive tract, and the morbidity and mortality rate of puppies are relatively high.
Principle of reaction] This product adopts rapid immunochromatographic technique to detect canine infectious hepatitis antigen in canine feces, oral, ocular and nasal secretions. Dilute the sample into a droplet of water.
After the diluted sample is dropped into the spiking hole, if ICH antigen exists in the sample, it will specifically bind to the colloidal gold labeled recombinant protein and form a complex along the layer.
binds and forms a complex that moves together along the chromatographic membrane and is captured by the membrane-coated ICH antibody, which shows a burgundy
red line on the detection line (T). If there is no ICH antigen in the sample, there is no visible line at the T position. Regardless of the negative or positive result, the C line should be visible.
result, the C line should be colored, otherwise the result is considered invalid.

Place in a cool and dry place away from light, store at 2-30℃, do not freeze, the use period of this product is 2 years.

Sample Requirements

This kit is only suitable for testing canine feces, oral, ocular and nasal secretion samples.
Fresh samples should be collected and put into the diluent immediately, and the diluted samples should be tested as soon as possible within 1 hour.
If the sample cannot be tested in time, it can be stored at 2-8℃ for 24 hours and -20℃ for 1 month.

1. Use a single-use sampler to collect samples of canine feces, oral, ocular and nasal secretions, put them into the dilution solution and mix thoroughly, remove the
   Remove the sampler, tighten the cap, mix upside down and let it stand for 1min.
2. Tear open the aluminum foil pouch of the test card, take out the test card and put it on a flat and clean table.
3. Pipette 3 drops (about 80μL) of the diluted sample into the spiked hole of the test card and wait for the result.
4. Wait for 10-15 minutes at room temperature to read the test result, the test result is invalid after more than 20 minutes.
   Note: The above reading time is carried out at room temperature 15-30 ℃, if the temperature is lower than 15 ℃, should be appropriate to extend the reading time; if the temperature is higher than 30 ℃, should be appropriate to extend the reading time; If the
   If the temperature is higher than 30℃, the reading time should be shortened appropriately.