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Pet testing kit is a tool used to detect the health status of pets. Here is a detailed introduction:
Common types
- Virus detection kit: Such as canine distemper virus antigen detection kit, canine parvovirus antigen rapid detection kit, feline panleukopenia detection test paper, etc. It can determine whether the pet is infected with the corresponding virus by detecting the virus antigens in the pet’s eye, nose, and secretions, feces, saliva, and other samples.
- Bacterial detection kit: Used to detect whether the pet is infected with specific bacteria, such as some kits that detect common bacteria such as Escherichia coli and Staphylococcus in pets, which can help veterinarians determine the source of infection to formulate corresponding treatment plans.
- Parasite detection kit: Commonly, there is a canine heartworm antigen detection kit, which detects whether the pet is infected with parasites by detecting the parasite antigens in the pet’s blood, providing a basis for pet deworming treatment.
- Disease marker detection kit: For example, canine C-reactive protein detection kit, canine total IgE detection kit, etc., it can detect specific markers in the pet’s blood to reflect the pet’s inflammatory state, immune state, etc., and assist veterinarians in comprehensively evaluating the pet’s health status.
Detection principle
- Immunochromatography: This is one of the most common detection principles in pet testing kits. Taking the canine distemper detection test paper as an example, the test paper strip contains specific antibodies against the canine distemper virus antigen. When the virus antigen in the sample combines with the antibody, a visible detection line will be formed on the test paper strip, and the result will be judged according to the presence or absence and color depth of the detection line.
- Enzyme-linked immunosorbent assay (ELISA): Such as the canine renin ELISA kit, using the specific binding reaction of antigens and antibodies, and detecting and analyzing the target substance in the sample through the color reaction of the enzyme-labeled antibody or antigen with the substrate, and measuring the absorbance value on the enzyme label meter, thereby performing quantitative or qualitative detection of the sample substance to be tested.
- PCR technology: Some kits for detecting viruses or bacteria use PCR technology, by amplifying specific gene fragments in the sample, and then detecting and analyzing the amplified products, which have high sensitivity and specificity, and can detect trace amounts of pathogen nucleic acids, such as the detection kit for Bartonella henselae.
Use method
- Sample collection: According to the detection target and the requirements of the kit, collect the corresponding samples, such as blood, feces, eye, nose, and secretions. During the collection process, it is necessary to use sterile instruments to avoid sample contamination.
- Sample processing: Some kits require processing of the collected samples, such as dilution, centrifugation, etc., to ensure that the substances to be tested in the sample can better react with the reagents.
- Detection operation: Follow the steps of the kit manual, add samples, reagents, etc. in turn, perform incubation, washing, and other operations. During the operation, it is necessary to strictly control the conditions such as time and temperature to ensure the accuracy of the detection result.
- Result interpretation: According to the instructions of the kit, determine the detection result by observing the color change of the test paper strip, the reading of the enzyme label meter, etc. The result interpretation methods of different kits may vary, and it is necessary to carefully read the instructions.
Characteristics and advantages
- Fast and convenient: Most pet testing kits are simple to operate and do not require complex instrument equipment, and can obtain detection results in a short time, which is convenient for pet owners to test at home and understand the pet’s health status in time.
- High specificity: For different pathogens or disease markers, the antibodies or primers in the kit have a high degree of specificity, and can accurately detect the target substance, reducing the occurrence of false positives or false negatives.
- High sensitivity: Especially the kits using PCR and other technologies can detect extremely low levels of pathogen nucleic acids, which is helpful for early detection of the pet’s infection situation and timely adoption of treatment measures.
- Non-invasive or minimally invasive: Such as the cat hematuria detection kit, just sprinkle the detection particles on the cat litter, and detect whether the cat has hematuria by observing the color change of the particles, without causing greater trauma to the pet, and the pet’s acceptance is high.
Precautions
- Choose regular products: There are many types of pet testing kits on the market, and the quality is uneven. It is recommended to choose products with quality assurance through regular channels to ensure the reliability of the detection results.
- Operate strictly according to the instructions: Before using the kit, be sure to read the instructions carefully and follow the prescribed steps and conditions for operation, otherwise, it may affect the accuracy of the detection result.
- Judge in combination with clinical symptoms: The detection result of the kit is only for reference, and the disease of the pet cannot be diagnosed only based on the detection result. If the detection result is positive or the pet shows abnormal symptoms, it should be promptly taken to the pet hospital for further examination and diagnosis to formulate an accurate treatment plan.
- Pay attention to preservation and expiration date: The kit should be stored under the specified conditions to avoid reagent failure. Before use, check the expiration date of the kit, and expired kits cannot be used.
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