Specific applications and characteristics of cell culture media are listed below:
Features
Nutrient-rich and adjustable: Contains a variety of essential nutrients, such as amino acids, vitamins, carbohydrates, inorganic salts, etc., which can meet the growth needs of different cell types. In addition, growth factors, hormones, serum and other components can be added according to the special needs of cells to achieve precise regulation of cell growth and differentiation, and promote cell proliferation, differentiation or expression of specific functions.
Stable pH and osmotic pressure: Usually equipped with buffer systems, such as sodium bicarbonate, HEPES, etc., which can effectively maintain the pH value of the culture medium in the range suitable for cell growth, generally around 7.2-7.4. At the same time, the osmotic pressure of the medium is also carefully adjusted to match the intracellular environment, which helps to maintain the normal morphology and physiological function of the cells and prevent the cells from being damaged due to osmotic pressure imbalance.
Sterility and exogenous contamination free: It undergoes strict aseptic treatment during production and use to avoid contamination by bacteria, fungi, mycoplasma and other microorganisms, ensuring a pure environment for cell culture. In addition, for serum-free media, etc., the quality of raw materials should be strictly controlled to avoid the introduction of exogenous proteins, viruses and other contaminants to ensure the safety and reliability of cell culture.
Strong customizability: According to different cell types, research purposes and experimental requirements, different types and formulations of cell culture media can be selected, or existing media can be improved and optimized by adding or removing specific components to meet individualized cell culture needs. For example, special media can be developed for certain difficult-to-cultivate cells, and there are corresponding media available for special needs such as serum-free culture and low-serum culture.
Supporting the growth of various cell types: from primary cells to various cell lines, from normal cells to tumor cells, from animal cells to human cells, etc., there are corresponding cell culture media that can support their growth and proliferation. Different types of cells have different requirements on the nutrients and growth factors of the culture media, and the rich variety of cell culture media can meet the specific needs of these different cell types, providing a wide range of options for cell biology research and application.
Strict quality control: High-quality cell culture media follow strict quality control standards during the production process, including the selection of raw materials, production process monitoring, finished product testing and other links. Each batch of media needs to be tested for a number of indicators, such as nutrient content, pH value, osmolality, sterility, endotoxin content, etc., to ensure the stability and consistency of the product quality, so as to ensure the reliability and reproducibility of the results of cell culture experiments.