The 96-well pre-filled kit has various applications in drug screening, which mainly include the following aspects:

Gene Expression Analysis
By extracting the nucleic acids of cells under different physiological or pathological states, and using the 96-well pre-filled kit in combination with technologies such as real-time quantitative PCR, the expression levels of a large number of genes can be detected in a high-throughput manner. When comparing the disease model with the normal model, genes with differential expression can be quickly screened out, and these genes may become potential drug targets.
Gene Function Research
Utilizing the RNA interference (RNAi) technology, conduct RNAi experiments on cells in a 96-well plate. Use the 96-well pre-filled kit to extract nucleic acids to verify the interference effect, and observe the phenotypic changes of cells, such as cell proliferation and apoptosis, after the gene expression is inhibited. Thus, the correlation between genes and diseases can be determined, providing a basis for the identification of drug targets.

Cell-level Screening
Add different compounds to the 96-well plate containing cultured tumor cells, virus-infected cells, etc. Use the 96-well pre-filled kit to extract the nucleic acids of the cells, and detect indicators such as the changes in the expression of genes related to the drug action and the copy number of viral nucleic acids. Quickly screen out the compounds that have an inhibitory or activating effect on the target cells or pathogens, and determine the preliminary activity of the drugs.
Enzyme Activity Screening
Many drug targets are enzymes. Enzymatic reactions can be carried out in a 96-well plate. After adding different compounds, use the 96-well pre-filled kit to extract the relevant nucleic acids to detect the changes in the expression of enzyme genes or the activity of enzymes, and screen out the drug molecules that can regulate the enzyme activity.

Genotoxicity Detection
Use the 96-well pre-filled kit to extract the nucleic acids of cells treated with drugs. By detecting the expression of genes related to DNA damage, the formation of micronuclei, DNA breaks, and other indicators, evaluate whether the drug has genotoxicity and determine the potential harm of the drug to the genetic material of cells.
Analysis of Metabolic Enzyme Gene Expression
The metabolism of drugs in the body is mainly completed by the cytochrome P450 and other metabolic enzyme systems. Use the 96-well pre-filled kit to detect the changes in the expression of metabolic enzyme genes in cells after drug treatment. If the expression of certain metabolic enzyme genes is abnormal, it may indicate that the drug will interfere with the normal metabolic process in the body, and there is a potential risk of toxicity.

Analysis of Signaling Pathways
Culture cells in a 96-well plate and treat them with drugs. Use the 96-well pre-filled kit to extract nucleic acids, and analyze the changes in the expression of genes related to the intracellular signaling pathways through technologies such as gene chips and RNA sequencing, to clarify the specific signaling pathways affected by the drugs and reveal the molecular mechanisms by which the drugs exert their effects.
Research on Epigenetic Regulation
Drugs may exert their effects through epigenetic mechanisms such as DNA methylation and histone modification. Use the 96-well pre-filled kit to extract the nucleic acids of cells, and detect the changes in relevant epigenetic markers, such as the level of DNA methylation and the degree of histone acetylation, to gain an in-depth understanding of the mechanism of action of drugs at the epigenetic level.