Gene Expression Analysis In biological research, researchers often use 96-well pre-filled kits for gene expression analysis. For example, when studying the differences between tumor cells and normal cells, RNA can be rapidly extracted from a large number of samples through this kit. Subsequently, it can be reverse-transcribed into cDNA for detecting the changes in gene expression levels by real-time quantitative PCR. This high-throughput method enables the simultaneous analysis of the expression of multiple genes in different samples, helping researchers understand the mechanisms of genes in processes such as cell growth, differentiation, and disease occurrence.
Pathogen Detection In the field of clinical diagnosis, 96-well pre-filled kits can be used for the nucleic acid detection of pathogens. Taking the detection of the novel coronavirus as an example, medical staff can use this kit to extract viral RNA from a large number of pharyngeal swab samples, and then perform PCR amplification for detection, enabling the rapid screening and diagnosis of the novel coronavirus. In addition, for other pathogens such as influenza virus, hepatitis B virus, and Mycobacterium tuberculosis, detection can also be carried out through similar methods, which is helpful for the early detection and treatment of diseases.
Research on SARS-CoV-2 In the in-depth research on SARS-CoV-2, the 96-well pre-filled kit plays an important role. Researchers can use it to efficiently extract nucleic acids from samples of cells or animal models infected with SARS-CoV-2, which can be used for analyzing the genomic sequence of the virus, studying the mutation situation of the virus, and exploring the interaction mechanism between the virus and host cells. Through the rapid processing of a large number of samples, the research process on SARS-CoV-2 can be accelerated, providing important basic data for vaccine development, drug screening, and so on.
Research on SARS-CoV The 96-well pre-filled kit also has great value for the research on SARS-CoV. In retrospective studies or the monitoring of potential risks of SARS-CoV, the kit can be used to extract nucleic acids from relevant samples for the analysis and detection of viral genes. It helps to deeply understand the transmission rules, pathogenic mechanisms of SARS-CoV, and the similarities and differences between it and other coronaviruses, providing a scientific basis and technical reserve for dealing with possible similar epidemics.
Genetic Disease Diagnosis In the diagnosis of genetic diseases, the 96-well pre-filled kit can be used to extract DNA from patients’ blood or tissue samples for gene detection and analysis. For example, for genetic diseases such as thalassemia and hemophilia, by detecting the mutations or deletions of related genes, it can provide important evidence for disease diagnosis, genetic counseling, and prenatal diagnosis. This high-throughput detection method can detect multiple gene loci simultaneously, improving the efficiency and accuracy of diagnosis.
Drug Development During the process of drug development, the 96-well pre-filled kit can be used for the research on the mechanism of drug action and the screening of drug targets. By extracting the nucleic acids of cells or animal models under different treatment conditions and analyzing the changes in the gene expression profile, the key genes and signaling pathways affected by the drug can be discovered, providing guidance for the design and optimization of drugs. At the same time, it can also be used to evaluate the effect of drugs on the nucleic acids of pathogens and screen drug compounds with antiviral, antibacterial, and other activities.
Forensic Identification In forensic physical evidence inspection, the 96-well pre-filled kit can be used to extract DNA from various biological samples (such as blood, hair, saliva, etc.) for individual identification, paternity testing, and so on. Its high-throughput characteristic enables the simultaneous processing of multiple samples, improving the efficiency and accuracy of identification, and providing powerful evidence support for solving criminal cases and resolving civil disputes.
Food Safety Detection In the field of food safety, the 96-well pre-filled kit can be used to detect pathogens in food, genetically modified components, etc. For example, it can detect pathogenic microorganisms such as Escherichia coli and Salmonella in food, as well as conduct qualitative and quantitative analysis of foreign genes in genetically modified foods to ensure the safety of consumers’ diet.