The 96-well preloaded kits are tools for extracting nucleic acids in batches, and they have important applications in multiple fields. The specific applications are as follows:

Disease Diagnosis: Nucleic acids can be extracted in batches from samples such as patients’ blood, saliva, and tissues. They are used to detect the nucleic acids of pathogens like viruses, bacteria, etc., such as the COVID-19 virus, hepatitis B virus, and Mycobacterium tuberculosis, assisting doctors in the diagnosis and differential diagnosis of infectious diseases. They can also be used to detect gene variations related to diseases, providing a basis for the diagnosis and classification of tumors and genetic diseases. For example, by detecting the nucleic acids of tumor tissues in lung cancer patients to determine whether there are specific gene mutations, it can guide the selection of targeted therapeutic drugs.
Disease Monitoring: During the treatment of diseases, the 96-well preloaded nucleic acid extraction kits are used to regularly extract patients’ nucleic acid samples to monitor changes in the nucleic acid load of pathogens or the level of gene expression, and evaluate the treatment effect and disease progression. For example, in the treatment of AIDS, the nucleic acid load of the HIV virus is monitored to judge the efficacy of antiviral treatment.

Gene Expression Analysis: RNA can be extracted in batches from cell and tissue samples for studying the differences in gene expression in different physiological states, developmental stages, or disease models. For example, the differences in the gene expression profiles between tumor cells and normal cells can be studied to find potential tumor markers or therapeutic targets.
Genomics Research: Genomic DNA of a large number of samples can be efficiently extracted for whole-genome sequencing, genotyping, genetic map construction, and other studies. For example, in the Human Genome Project, this type of kit was used to extract genomic DNA from different individuals in batches to provide high-quality samples for genome sequencing.
Molecular Cloning and Genetic Engineering: The extracted nucleic acids can be used as templates for PCR amplification, gene cloning, and other experiments to construct recombinant DNA molecules for expressing specific proteins or studying the functions of genes. For example, an expression vector containing the target gene can be constructed and transfected into cells for protein expression and function studies.

Crop Breeding: Nucleic acids can be extracted from plant tissue samples for analyzing the gene diversity and genetic purity of crops, assisting in the breeding and improvement of crop varieties. For example, through the analysis of nucleic acids of different rice varieties, varieties with excellent traits can be screened out for cultivation.
Pest and Disease Detection: Nucleic acids can be extracted from plant samples infected with pests and diseases or insect samples to detect the types and quantities of pathogens or pests, providing a basis for the prevention and control of pests and diseases. For example, the nucleic acids of the pathogen causing citrus Huanglongbing can be detected to discover and prevent the spread of the disease in a timely manner.

Microbial Detection: Nucleic acids of microorganisms can be extracted from food samples to quickly detect the contamination of bacteria, viruses, fungi, and other microorganisms in food. For example, Staphylococcus aureus in milk and Salmonella in meat can be detected to ensure food safety.
Genetically Modified Detection: They are used to extract nucleic acids in food to detect whether there are genetically modified components, providing technical support for food safety supervision and consumers’ right to know. For example, genetically modified components in genetically modified soybean oil and corn products can be detected to ensure that the products comply with relevant regulations and standards.