The following is a general method for preparing virus preservation liquid:

Prepare materials and equipment

• Materials: phosphate buffer salts (such as potassium dihydrogen phosphate, dipotassium hydrogen phosphate), sodium chloride, bovine serum albumin, glycerol, EDTA, penicillin, streptomycin, and other required reagents according to specific needs.
• Equipment: analytical balance, magnetic stirrer, pH meter, volumetric flask, beaker, pipette, etc.

Preparation steps

1. Prepare buffer solution: Weigh an appropriate amount of phosphate buffer salts (for example, 0.24 g of potassium dihydrogen phosphate and 1.44 g of dipotassium hydrogen phosphate) and dissolve them in about 800 mL of distilled water. Use a magnetic stirrer to stir until completely dissolved. Then add 8.5 g of sodium chloride and continue to stir until dissolved.
2. Adjust pH value: Use a pH meter to measure the pH value of the buffer solution and adjust it to 7.2-7.4 with hydrochloric acid or sodium hydroxide solution as needed.
3. Add other components: Add 0.5-1.0 g of bovine serum albumin and 200-300 mL of glycerol to the buffer solution. Stir evenly to make them fully dissolved. Then add an appropriate amount of EDTA (usually 0.01-0.02 mol/L) and continue to stir.
4. Add antibacterial agents: Add penicillin and streptomycin to make their final concentrations reach 100-200 U/mL and 100-200 μg/mL respectively to prevent bacterial contamination.
5. Dilute and adjust: Add distilled water to the solution to make the final volume reach 1000 mL. Stir evenly to ensure that all components are evenly distributed.
6. Sterilization: Filter the prepared virus preservation liquid through a 0.22 μm filter membrane for sterilization to remove possible bacteria and impurities and ensure the sterility of the preservation liquid.
7. Quality inspection: After sterilization, take a small amount of the preservation liquid for quality inspection, such as testing its pH value, osmotic pressure, and sterility to ensure that it meets the requirements for virus preservation.

It should be noted that the above formula and operation steps are for reference only. In actual production and research, it may need to be adjusted and optimized according to different virus types and specific application requirements. In addition, when handling biological reagents, pay attention to strict aseptic operation and personal protection to avoid potential biological risks.

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