Transport media are essentially solutions of buffers with carbohydrates, peptones and other nutrients (excluding growth factors)
designed to preserve the viability of bacteria during transport without allowing their multiplication.
The primary objective of the use of the transport medium is to maintain the specimen as near its original state as possible.
Transport medium aims to preserve a specimen and minimize bacterial overgrowth from the time of collection to the processing of the specimen.
Depending on the type of organisms suspect in the sample, transport media may vary.
However, in general, transport media are classified on the basis of the physical state as semi-solid and liquid and also on the basis of their utility as bacterial or viral transport media.
What does transport media contain?
What samples are collected in transport medium?
All types of samples that may contain pathogens but could not be process immediately requires a transport medium.
It may be Stool, urethral swabs, Nasal and throat swabs and specimens for tissue culture etc.
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Given below are some examples of transport medium with their purpose:
Cary and Blair Medium: semi-solid, white color transport medium for faeces that may contain Salmonella, Shigella, Vibrio or Campylobacter
Amies medium with charcoal:
Charcoal helps eliminate metabolic products of bacterial growth, which may be especially useful in the isolation of fastidious organisms like.
However it is suggested that, some other pathogens like Campylobacter can also survive in such medium.
Amies medium without charcoal:
Are ideal for the isolation of Mycoplasma and Ureaplasma
Commonly used for transporting specimens suspecte of having gonococci.
Also use for transporting Throat, wound and skin swabs that may contain fastidious organisms.
Venkatraman Ramakrishnan (VR) medium:
Use to transport feces from suspecte cholera patients.
Sach’s buffered glycerol saline:
Used to transport faeces from patients suspecte to be suffering from bacillary dysentery.
Viral Transport Medium:
Viral Transport Medium (VTM) is ideal for diagnosis of viral infection.
Ocular, respiratory and tissue swabs can be submit in this medium.
Fluid samples such as tracheal wash specimens or peritoneal fluid should be submit as is, in sterile vials which prevent desiccation.
In the absence of viral transport medium, submit swabs in sterile, sealed vials with several drops of saline add, to prevent desiccation.
Cotton, plastic, wood-handled, and Dacron and other synthetic swabs are all acceptable.
Calcium alginate swabs should be avoided. Bacterial transport media are not appropriate for virology.
Anaerobic Transport Medium (ATM):
It is a kind of mineral salt semi-solid medium containing reducing agent, which is design to maintain the activity of anaerobic bacteria.
It contains not only buffer mineral salts, but also sodium mercaptoacetate and cysteine to provide a reducing environment.
Resazurin may also be add as a redox indicator to reveal exposure to oxygen by turning pink.
It provides an environment, which maintains the viability of most microorganisms without significant multiplication and allows for dilution of inhibitors present in clinical material. Examples include thioglycolate broth
VTM Swab Kit with a nasal swab
VTM Swab Kit with an oral swab
VTM Swab Kit with 2 swabs ( nasal swab+oral swab)
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HCY takes the product quality as the essence of enterprise development, adhering to the principle of “first-class products, first-class services” in an all-round way, pursuing the enterprise spirit of “seeking truth, innovation, unity, and efficiency”. HCY organizes the whole process of production and sales in strict accordance with ISO9001 and ISO13485 management system, with stable performance and reliable quality.